Biocrede’s Ultrataq DNA Polymerase is a gold standard in next-generation DNA polymerase enzymes. Now you can streamline your PCR with Biocrede’s Ultrataq 2x MasterMix with dye. Biocrede’s 2x PCR MasterMix with dye provides all of the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR process. This MasterMix comes with an inert blue dye and a stabilizer that allow for loading of the pcr products directly into an agarose gel, streamlining the process and reducing the risk of contamination.
-Robust and high yield PCR across an array of templates.
-High processivity and shortened extension period.
-High fidelity, over 50x better than taq polymerase.
-Low cost per unit against comparable enzymes.
-Efficient amplification up to 16 kb.
-Robust amplification of AT and GC rich templates.
-Amplification of long or difficult amplicons
-AT and GC rich templates
-Template generation for NGS library construction
Picture 1: Ultrataq DNA Polymerase successfully amplifies genomic template up to 15.6 kb.
|Polymerase||Target Length||Extension Speed (per min.)||Fidelity (vs. Taq)||Proofreading||MasterMix|
|Ultrataq||15 kb||3-4 kb||50x||Yes||Yes|
|Exactitaq||6 kb||1 kb||60x||Yes||Yes|
|Taq||6 kb||1 kb||1x||No||Yes|
|Hottaq||6 kb||1 kb||1x||No||Yes|
|Infinitaq||11 kb||4-6 kb||10x||Yes||Yes|
|Hawkeye||20 kb||3-4 kb||1x||No||Yes|
|Hemotaq||1 kb||1 kb||1x||No||Yes|
Concentration: 5 U/μl
Storage: Store all components at -20 °C.Notes
Recommended products: UltraTaq master mix, UltraTaq master mix w/ dye, dNTP, Safe DNA stains
Notes: This product is distributed for laboratory research only.
How can I optimize the reaction for multiplexing?
The polymerase and Mg concentrations need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.
Is UltraTaq DNA Polymerase compatible with Uracil?
UltraTaq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.
If no amplification products are obtained, what parameters should be considered first when troubleshooting?
First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.
If there is still no product the PCR conditions might be too stringent and should be changed as follows:
The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich.
If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem. Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.
Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?
No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on UltraTaq PCR products, you can clean up the UltrTaq PCR reaction first, then mix the cleaned-up UltraTaq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the UltraTaq PCR products.
Biocrede's Exactitaq is an industry-leading high-fidelity DNA polymerase. Biocrede's 2X PCR MasterMix provides all the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Exactitaq is the ideal choice for PCR that requires increased accuracy like cloning and sequencing, eliminating the need for downstream error correction steps. The use of Exactitaq DNA Polymerase for rare DNA templates is necessary as small amounts of DNA are especially prone to high mutant frequencies.
• Whole genome sequencing
• Site-directed mutagenesis
• Blunt-end cloning
• Specific amplification of difficult templates (i.e. GC-Rich)
• Low copy PCR assays
Form 5 x 1.0 ml vials
Storage Store all components at -20 °C.
Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
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