Second-Strand cDNA Synthesis Kit

Biocrede Inc.

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Product Description

Biocrede’s Second Strand cDNA Synthesis Kit is an efficient system of generating double stranded cDNA from first strand cDNA as a template. The E. coli RNase H nicks the RNA in the DNA:RNA hybrid, while the E. coli DNA Polymerase replaces the RNA with deoxyribonucleotides. The E. coli DNA Ligase completes the double stranded DNA formation by linking the gaps between the newly synthesized cDNA strand. The dNTP based kit and the dNTP/dUTP based kit offer different combinations of deoxyribonucleotides tailored to the end user’s needs and application.



  • RNA-Seq Library Construction.
  • downstream double-stranded blunt-end cDNA synthesis for cloning.
  • downstream double-stranded cDNA library construction.

Protocol  Click To Open


  • one enzyme unit is defined as the amount of enzyme required to incorporate 1 nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37oC using Poly(A) and Oligo(dT) as template and primer, respectively.
  • Noted that these enzymes may still contain E. coli gDNA.
  • Reaction Buffer Components: 20 mM Tris-HCl (pH 7.5), 12 mM (NH4)2SO4, 10 mM MgCl2, 0.16 mM β-NAD.

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About Us

Biocrede MLSIC


Biocrede was established in June of 2012 (Ann Arbor, MI, USA) and is dedicated to transforming lives via innovative biomed-tech solutions that restore health, extend life, and reduce hospitalization length for all types of patients in the world, via comprehensive research, design, development, manufacturing and customer service in the eyes of our customers, employees, competitors, and healthcare communities.  We are currently focusing on life sciences products to deliver custom viral vectors, pcr, diagnostics tools, and the research, development and commercialization of next generation medical solutions.



We believe our responsibility priority is to doctors, nurses, and patients, and thus we develop solutions, supply products, and provide services with high quality and ethics.  We are responsible to our employees, and it is our desire to see every employee achieve their highest potential by striving to provide career counseling, appropriate training, job required education, and guidance with respect, dignity and merit recognition.  We strive to play an integral role in to the society and the communities we live by creating value to help people globally have healtheir and more fulfilling lives, and make decisions that protect the environment and natural resources in the world. Our final responsibility is to our stockholders by making the business profitable.