Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with Biocrede’s RTScript Plus Reverse Transcriptase. Engineered to perform under high temperatures (45°C - 55°C), Biocrede’s RTScript Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, RTScript Plus Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.
Biocrede's One-Step RT-PCR Kit contains all necessary reagents for both reverse transcription and PCR amplification to occur in a single reaction tube. Specifically, this One-Step RT-PCR kit contains RTase and UltraTaq DNA Polymerase in a convenient format for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-PCR buffer contains stabilizers and enhancers that optimize the two reactions in a “single step”. Together with a specially formulated RT-PCR buffer, this One-Step RT-PCR kit offers the end-users an efficient, easy to use and reliable alternative to conventional “two-step” sequential RT-PCR.View full product details
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