QuantiGreen miRNA 2X qPCR MasterMix iCycler

Biocrede Inc.

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Product Description

Biocrede’s QuantiGreen miRNA 2X qPCR MasterMix iCycler is designed specifically for quantitative real-time analysis of miRNA samples. The components of QuantiGreen miRNA 2X qPCR MasterMix iCycler have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified HotTaq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.

Storage               

Upon arrival,  QuantiGreen miRNA 2X qPCR MasterMix should be stored at -20ºC and protected from light. After each experiment, the leftover thawed mix can be stored at 4ºC if it is to be used within the next 3 months.

Notes   

Primer concentration should not be high. 100nM to 300nM each primer usually gives best results

- Melting curve is useful in distinguishing nonspecific amplification products and primer dimers from desired product. It's recommended to run melting curve following amplification cycles.

- To do replicates

- To reduce pipetting error: preparing amplicon specific master mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range are very effective to get tight Ct.



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About Us


Biocrede MLSIC

COMPANY OVERVIEW/MISSION

Biocrede was established in June of 2012 (Ann Arbor, MI, USA) and is dedicated to transforming lives via innovative biomed-tech solutions that restore health, extend life, and reduce hospitalization length for all types of patients in the world, via comprehensive research, design, development, manufacturing and customer service in the eyes of our customers, employees, competitors, and healthcare communities.  We are currently focusing on life sciences products to deliver custom viral vectors, pcr, diagnostics tools, and the research, development and commercialization of next generation medical solutions.

 

 

OUR CREDO

We believe our responsibility priority is to doctors, nurses, and patients, and thus we develop solutions, supply products, and provide services with high quality and ethics.  We are responsible to our employees, and it is our desire to see every employee achieve their highest potential by striving to provide career counseling, appropriate training, job required education, and guidance with respect, dignity and merit recognition.  We strive to play an integral role in to the society and the communities we live by creating value to help people globally have healtheir and more fulfilling lives, and make decisions that protect the environment and natural resources in the world. Our final responsibility is to our stockholders by making the business profitable.