Biocrede’s QuantiGreen miRNA 2X qPCR MasterMix iCycler is designed specifically for quantitative real-time analysis of miRNA samples. The components of QuantiGreen miRNA 2X qPCR MasterMix iCycler have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified HotTaq polymerase included in our MasterMix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Upon arrival, QuantiGreen miRNA 2X qPCR MasterMix should be stored at -20ºC and protected from light. After each experiment, the leftover thawed mix can be stored at 4ºC if it is to be used within the next 3 months.
Primer concentration should not be high. 100nM to 300nM each primer usually gives best results
- Melting curve is useful in distinguishing nonspecific amplification products and primer dimers from desired product. It's recommended to run melting curve following amplification cycles.
- To do replicates
- To reduce pipetting error: preparing amplicon specific master mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range are very effective to get tight Ct.
Amplify difficult DNA targets in real-time PCR with Biocrede’s hiGC-2ndStructure 2X qPCR MasterMix. Biocrede’s hiGC-2ndStructure 2X qPCR MasterMix is designed for amplification of complex DNA fragments (i.e. high GC content and secondary structure) ranging from 300bp to 1.3kb in length. Available with the option of ROX or fluorescein as the internal passive reference dye, Biocrede’s hiGC-2ndStructure 2X qPCR MasterMix enables reliable amplification and offers unmatched performance in sensitivity and signal-to-noise ratio.
Biocrede's hiGC-2ndStructure 2X qPCR MasterMix is supplied as 4 x 1.25ml AliquotsView full product details
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