Convenient real-time RNA quantification in one EASY step. Biocrede’s One-Step TaqTicProbe qRT-PCR iCycler Kit uses a combination of high-quality enzymes in a proprietary buffer system to deliver precise and accurate sample analysis for high-throughput applications. This kit offers ultimate convenience in addition to consistent performance in terms of high sensitivity and superb signal-to noise ratio.
While a one-step/single tube setup provides overall convenience and reduces room for error, Biocrede’s One-Step TaqTicProbe qRT-PCR iCycler Kit offers additional advantages:
- Improved fidelity and yield for reverse transcription
- Prevention of template (RNA) degradation with RNaseRID Ribonuclease Inhibitor.
- Superb performance with respect to sensitivity and signal-to-noise ratio.
- Significant reduction in non-specific PCR amplification by utilizing HotTaq DNA polymerase in the enzyme mix.
Key Features
- Streamlined protocol in a simple, single-tube reaction set-up
- High-quality, full-length cDNA from as little as 0.01pg of RNA template
- Fully optimized for detection of low-copy genes
- Simple set-up for any RNA template (e.g. mRNA and total RNA)
- Reduces pipetting steps to minimize the risk of contamination and error.
Applications
- Gene-expression analysis
- Transcription analysis
- Gene cloning
- High throughput applications
- Virus detection and quantification
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hiGC-2ndStructure 2X qPCR MasterMix
$125.00
Product Description
Amplify difficult DNA targets in real-time PCR with Biocrede’s hiGC-2ndStructure 2X qPCR MasterMix. Biocrede’s hiGC-2ndStructure 2X qPCR MasterMix is designed for amplification of complex DNA fragments (i.e. high GC content and secondary structure) ranging from 300bp to 1.3kb in length. Available with the option of ROX or fluorescein as the internal passive reference dye, Biocrede’s hiGC-2ndStructure 2X qPCR MasterMix enables reliable amplification and offers unmatched performance in sensitivity and signal-to-noise ratio.
Key Features
- Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments
Application
- Next generation sequencing
- Gene-expression analysis
- Gene knockdown validation
- SNP genotyping assays
- CHiP
- Copy number variation
- Microarray validation
- High throughput applications
- Virus detection and quantification
Biocrede's hiGC-2ndStructure 2X qPCR MasterMix is supplied as 4 x 1.25ml Aliquots
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