One-Step TaqTicProbe qRT-PCR iCycler

Convenient real-time RNA quantification in one EASY step. Biocrede’s One-Step TaqTicProbe qRT-PCR iCycler Kit uses a combination of high-quality enzymes in a proprietary buffer system to deliver precise and accurate sample analysis for high-throughput applications. This kit offers ultimate convenience in addition to consistent performance in terms of high sensitivity and superb signal-to noise ratio.

While a one-step/single tube setup provides overall convenience and reduces room for error, Biocrede’s One-Step TaqTicProbe qRT-PCR iCycler Kit offers additional advantages:

• Improved fidelity and yield for reverse transcription
• Prevention of template (RNA) degradation with RNaseRID Ribonuclease Inhibitor.
• Superb performance with respect to sensitivity and signal-to-noise ratio.
• Significant reduction in non-specific PCR amplification by utilizing HotTaq DNA polymerase in the enzyme mix.

Key Features

• Streamlined protocol in a simple, single-tube reaction set-up
• High-quality, full-length cDNA from as little as 0.01pg of RNA template
• Fully optimized for detection of low-copy genes
• Simple set-up for any RNA template (e.g. mRNA and total RNA)
• Reduces pipetting steps to minimize the risk of contamination and error.

Applications

• Gene-expression analysis
• Transcription analysis
• Gene cloning
• High throughput applications
• Virus detection and quantification