Product Description
HotTaq DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specifi c product formations that would otherwise competev for reagent availability. abm’s HotTaq DNA Polymerase offers higher specificity and improved yield of PCR products. PCR products amplifi ed up to 6 kb in length with HotTaq Taq DNA Polymerase contain a single base (A) 3’ overhang.
Features and Benefits
- Saves preparation time by combining HotTaq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture.
- Reduces the risk of contamination by decreasing the number of pipetting steps.
- Provides consistent reaction performance and results.
Related Items
100bp DNA Marker
$48.00
Description
Biocrede's 100 bp DNA marker has been optimized for imaging alongside PCR products made from Biocrede Mastermixes or other SYBR Green-like PCR products.
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA. The bands range from 50 bp to 1.5 kb and show increased intensity at the 200 bp and 500 bp bands. The approximate mass of each band is provided for estimating the mass of DNA in comparison to DNA samples of similar size.
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