Product DescriptionBiocrede's 2X PCR HotTaq MasterMix is a ready-to-use mixture of high quality hot start DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for amplification of DNA. HotTaq DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, Biocrede’s HotTaq DNA Polymerase offers improved yield of desired PCR products.
The 2X PCR HotTaq MasterMix with dye contains, in addition, an inert green dye blend and a stabilizer which allows for direct loading of the final products onto a gel for analysis. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.
Features and Benefits
Biocrede's Exactitaq is an industry-leading high-fidelity DNA polymerase. Biocrede's 2X PCR MasterMix provides all the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Exactitaq is the ideal choice for PCR that requires increased accuracy like cloning and sequencing, eliminating the need for downstream error correction steps. The use of Exactitaq DNA Polymerase for rare DNA templates is necessary as small amounts of DNA are especially prone to high mutant frequencies.
• Whole genome sequencing
• Site-directed mutagenesis
• Blunt-end cloning
• Specific amplification of difficult templates (i.e. GC-Rich)
• Low copy PCR assays
Form 5 x 1.0 ml vials
Storage Store all components at -20 °C.
Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
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