Biocrede's HiTaq DNA Polymerase is a novel DNA polymerase with strategically engineered mutations resulting in a robust, high-fidelity polymerase. HiTaq DNA polymerase has exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR.
Biocrede's HiTaq 2X PCR MasterMix is a ready-to-use mixture containing all the necessary reagents for highly successful amplification of DNA (high-fidelity DNA Polymerase, deoxynucleotides, reaction buffer and a sophisticated blend of additives in a 2X concentration). The HiTaq 2X PCR MasterMix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. HiTaq 2X PCR MasterMix is the most robust PCR MasterMix commercially available and will deliver an exceptional product yield with high fidelity.
HiTaq 2X PCR MasterMix with dye contains an inert green dye mixture and a stabilizer which allow for direct loading of the final PCR products onto an agarose gel for analysis. The green dye resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of the gel electrophoresis, and the yellow band indicates the migrating front of the gel.
Features and Benefits
Biocrede's 100 bp DNA marker has been optimized for imaging alongside PCR products made from Biocrede Mastermixes or other SYBR Green-like PCR products.
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA. The bands range from 50 bp to 1.5 kb and show increased intensity at the 200 bp and 500 bp bands. The approximate mass of each band is provided for estimating the mass of DNA in comparison to DNA samples of similar size.
View full product details
Biocrede was established in June of 2012 (Ann Arbor, MI, USA) and is dedicated to transforming lives via innovative biomed-tech solutions that restore health, extend life, and reduce hospitalization length for all types of patients in the world, via comprehensive research, design, development, manufacturing and customer service in the eyes of our customers, employees, competitors, and healthcare communities. We are currently focusing on life sciences products to deliver custom viral vectors, pcr, diagnostics tools, and the research, development and commercialization of next generation medical solutions.
We believe our responsibility priority is to doctors, nurses, and patients, and thus we develop solutions, supply products, and provide services with high quality and ethics. We are responsible to our employees, and it is our desire to see every employee achieve their highest potential by striving to provide career counseling, appropriate training, job required education, and guidance with respect, dignity and merit recognition. We strive to play an integral role in to the society and the communities we live by creating value to help people globally have healtheir and more fulfilling lives, and make decisions that protect the environment and natural resources in the world. Our final responsibility is to our stockholders by making the business profitable.