DNase I is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.
- Removes DNA from protein preparations and RNA samples
- Mediates nick translation
- Generates random fragments for dideoxy sequencing
- Degrade template DNA following in vitro transcription
- Mediate DNase I foot-printing
Storage 10 mM Tris-HCl (pH 7.6), 2 mM CaCl2, and 50% (v/v) Glycerol.
Handling Store all components at -20°C.
Notes One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.
This product is distributed for laboratory research only Caution: Not for diagnostic use
Biocrede's 100 bp DNA marker has been optimized for imaging alongside PCR products made from Biocrede Mastermixes or other SYBR Green-like PCR products.
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA. The bands range from 50 bp to 1.5 kb and show increased intensity at the 200 bp and 500 bp bands. The approximate mass of each band is provided for estimating the mass of DNA in comparison to DNA samples of similar size.
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Biocrede was established in June of 2012 (Ann Arbor, MI, USA) and is dedicated to transforming lives via innovative biomed-tech solutions that restore health, extend life, and reduce hospitalization length for all types of patients in the world, via comprehensive research, design, development, manufacturing and customer service in the eyes of our customers, employees, competitors, and healthcare communities. We are currently focusing on life sciences products to deliver custom viral vectors, pcr, diagnostics tools, and the research, development and commercialization of next generation medical solutions.
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