Biocrede's non-toxic dyes represent a new and safe class of nucleic acid stains for the visualization of DNA in agarose and polyacrylamide gels. The dyes are developed to replace Ethidium Bromide, a potent mutagen, commonly used in gel electrophoresis for visualization of nucleic acids in agarose and polyacrylamide gels. This bundle comes with green, white, and red dyes that are all easy-to-use and non-toxic.
-Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.
Biocrede's 100 bp DNA marker has been optimized for imaging alongside PCR products made from Biocrede Mastermixes or other SYBR Green-like PCR products.
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA. The bands range from 50 bp to 1.5 kb and show increased intensity at the 200 bp and 500 bp bands. The approximate mass of each band is provided for estimating the mass of DNA in comparison to DNA samples of similar size.
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