Looking to improve your routine PCR? UltraTaq DNA Polymerase is the new standard. With outstanding PCR yield, exceptional fidelity and high processivity, Biocrede’s UltraTaq DNA Polymerase is a versatile enzyme ideal for all PCR applications. With this superior enzyme, you can consolidate all PCR protocols and reactions into one efficient system.
With Biocrede’s UltraTaq DNA Polymerase, you can expect
Picture 1: Ultrataq DNA Polymerase successfully amplifies genomic template up to 15.6 kb.
|Polymerase||Target Length||Extension Speed (per min.)||Fidelity (vs. Taq)||Proofreading||MasterMix|
|Ultrataq||15 kb||3-4 kb||50x||Yes||Yes|
|Exactitaq||6 kb||1 kb||60x||Yes||Yes|
|Taq||6 kb||1 kb||1x||No||Yes|
|Hottaq||6 kb||1 kb||1x||No||Yes|
|Infinitaq||11 kb||4-6 kb||10x||Yes||Yes|
|Hawkeye||20 kb||3-4 kb||1x||No||Yes|
|Hemotaq||1 kb||1 kb||1x||No||Yes|
Concentration: 5 U/μl
Storage: Store all components at -20 °C.Notes
Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stainsIs Ultrataq DNA Polymerase compatible with Uracil?
Ultrataq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.
If no amplification products are obtained, what parameters should be considered first when troubleshooting?
First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.
If there is still no product the PCR conditions might be too stringent and should be changed as follows:
The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich.
If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem. Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.
Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?
No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on ultrataq PCR products, you can clean up the ultrataq PCR reaction first, then mix the cleaned-up ultrataq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the bestaq PCR products.
Biocrede's 100 bp DNA marker has been optimized for imaging alongside PCR products made from Biocrede Mastermixes or other SYBR Green-like PCR products.
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA. The bands range from 50 bp to 1.5 kb and show increased intensity at the 200 bp and 500 bp bands. The approximate mass of each band is provided for estimating the mass of DNA in comparison to DNA samples of similar size.
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Biocrede was established in June of 2012 (Ann Arbor, MI, USA) and is dedicated to transforming lives via innovative biomed-tech solutions that restore health, extend life, and reduce hospitalization length for all types of patients in the world, via comprehensive research, design, development, manufacturing and customer service in the eyes of our customers, employees, competitors, and healthcare communities. We are currently focusing on life sciences products to deliver custom viral vectors, pcr, diagnostics tools, and the research, development and commercialization of next generation medical solutions.
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