Convenient and time-saving reaction set-up with Biocrede’s RTScript Two-Step RT-PCR Kit for complex RNA templates (i.e. secondary structures and high GC content). This highly efficient two-step RT-PCR kit consists of two main components: RTScript Plus cDNA Synthesis SuperMix and TaqPlus 2X PCR MasterMix with dye.
Biocrede’s cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, Biocrede’s cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. RTScript Plus is engineered to perform under high temperatures (45°C - 55°C), Biocrede’s RTScript Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length.
Biocrede’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, Biocrede offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. Biocrede’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
Biocrede's One-Step RT-PCR Kit contains all necessary reagents for both reverse transcription and PCR amplification to occur in a single reaction tube. Specifically, this One-Step RT-PCR kit contains RTase and UltraTaq DNA Polymerase in a convenient format for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-PCR buffer contains stabilizers and enhancers that optimize the two reactions in a “single step”. Together with a specially formulated RT-PCR buffer, this One-Step RT-PCR kit offers the end-users an efficient, easy to use and reliable alternative to conventional “two-step” sequential RT-PCR.View full product details
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