From RNA template to cDNA in just 15 minutes completely hassle-free. With Biocrede’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of our MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience.
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
Biocrede's One-Step RT-PCR Kit contains all necessary reagents for both reverse transcription and PCR amplification to occur in a single reaction tube. Specifically, this One-Step RT-PCR kit contains RTase and UltraTaq DNA Polymerase in a convenient format for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-PCR buffer contains stabilizers and enhancers that optimize the two reactions in a “single step”. Together with a specially formulated RT-PCR buffer, this One-Step RT-PCR kit offers the end-users an efficient, easy to use and reliable alternative to conventional “two-step” sequential RT-PCR.View full product details
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