2X One-Step RT-PCR Buffer

5X All-In-One MasterMix (DNA decontamination kit)

$160.00

Product Description

Removal of contaminating genomic DNA from your samples to reverse transcription of RNA in 30 minutes, with 5X All-In-One RT MasterMix (RTScript Genomic DNA Removal Kit included). Biocrede’s 5X All-in-One RT MasterMix (with RTScript Genomic DNA Removal Kit) offers a convenient and complete system to facilitate the complete elimination of contaminating genomic DNA in RNA preparations for rapid synthesis of high-quality cDNA for downstream applications.

Key Features

• Efficient ds and ssDNA digestion in under 10 minutes
• Maximal RNA protection – No heating or organic extraction steps required
• Fewer handling errors, with only 1 liquid transfer step for reverse transcription
• Streamlined protocol suitable for high-throughput applications
• Simple set-up for any RNA template
• High reproducibility and excellent cDNA yield

Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.

Application

• Generation of templates for use in RT-PCR and qRT-PCR
• cDNA synthesis from ssRNA
• cDNA library construction
• Generation of probes for hybridization
• DNA primer extension