Biocrede's One-Step RT-PCR Kit contains all necessary reagents for both reverse transcription and PCR amplification to occur in a single reaction tube. Specifically, this One-Step RT-PCR kit contains RTase and UltraTaq DNA Polymerase in a convenient format for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-PCR buffer contains stabilizers and enhancers that optimize the two reactions in a “single step”. Together with a specially formulated RT-PCR buffer, this One-Step RT-PCR kit offers the end-users an efficient, easy to use and reliable alternative to conventional “two-step” sequential RT-PCR.
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5X All-In-One MasterMix (DNA decontamination kit)
$160.00
Product Description
Removal of contaminating genomic DNA from your samples to reverse transcription of RNA in 30 minutes, with 5X All-In-One RT MasterMix (RTScript Genomic DNA Removal Kit included). Biocrede’s 5X All-in-One RT MasterMix (with RTScript Genomic DNA Removal Kit) offers a convenient and complete system to facilitate the complete elimination of contaminating genomic DNA in RNA preparations for rapid synthesis of high-quality cDNA for downstream applications.
Key Features
- Efficient ds and ssDNA digestion in under 10 minutes
- Maximal RNA protection – No heating or organic extraction steps required
- Fewer handling errors, with only 1 liquid transfer step for reverse transcription
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent cDNA yield
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
Application
- Generation of templates for use in RT-PCR and qRT-PCR
- cDNA synthesis from ssRNA
- cDNA library construction
- Generation of probes for hybridization
- DNA primer extension
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