Biocrede's One-Step RT-PCR Kit contains all necessary reagents for both reverse transcription and PCR amplification to occur in a single reaction tube. Specifically, this One-Step RT-PCR kit contains RTase and UltraTaq DNA Polymerase in a convenient format for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-PCR buffer contains stabilizers and enhancers that optimize the two reactions in a “single step”. Together with a specially formulated RT-PCR buffer, this One-Step RT-PCR kit offers the end-users an efficient, easy to use and reliable alternative to conventional “two-step” sequential RT-PCR.
Related Items
5X All-In-One MasterMix (DNA decontamination kit)
$160.00
Product Description
Removal of contaminating genomic DNA from your samples to reverse transcription of RNA in 30 minutes, with 5X All-In-One RT MasterMix (RTScript Genomic DNA Removal Kit included). Biocrede’s 5X All-in-One RT MasterMix (with RTScript Genomic DNA Removal Kit) offers a convenient and complete system to facilitate the complete elimination of contaminating genomic DNA in RNA preparations for rapid synthesis of high-quality cDNA for downstream applications.
Key Features
- Efficient ds and ssDNA digestion in under 10 minutes
- Maximal RNA protection – No heating or organic extraction steps required
- Fewer handling errors, with only 1 liquid transfer step for reverse transcription
- Streamlined protocol suitable for high-throughput applications
- Simple set-up for any RNA template
- High reproducibility and excellent cDNA yield
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
Application
- Generation of templates for use in RT-PCR and qRT-PCR
- cDNA synthesis from ssRNA
- cDNA library construction
- Generation of probes for hybridization
- DNA primer extension
Biocrede Inc.
Sign up to get the latest on sales, new releases and more …
© 2017 Biocrede Inc..
All Rights Reserved. Reproduction of any materials from the site is strictly forbidden without permission. Biocrede Inc. brand products are sold exclusively through Biocrede Inc.
Powered by Shopify
