100bp DNA Marker

$48.00

Biocrede's 100 bp DNA marker has been optimized for imaging alongside PCR products made from Biocrede Mastermixes or other SYBR Green-like PCR products. 

PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA. The bands range from 50 bp to 1.5 kb and show increased intensity at the 200 bp and 500 bp bands. The approximate mass of each band is provided for estimating the mass of DNA in comparison to DNA samples of similar size. 

Protocol  

100bp DNA Marker Non-Toxic - Green

$78.00

Description

Biocrede's 100 bp Non-Toxic DNA Marker is a non-toxic and efficient alternative DNA visualizing agent to ethidium bromide, a potent carcinogen. The brightness is optimized for compatible and proper camera exposure in applications along with PCR products generated from Biocrede Master Mixes as well as from any other SYBR Green-alike-based PCR amplification. Simply load 5 ul of the marker onto a plain gel and perform electrophoresis along with samples; Visualize the gel using an UV light source. 

PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA.

Protocol  

100bp DNA Marker Plus

$48.00

Description

PCR products and double-stranded DNA were digested  to yield 12 bands, ranging from 100 bp – 3 kb, for molecular weight standards in agarose gel electrophoresis. The 500 and 1,500 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

Protocol  

1kb DNA Marker

$48.00

Description

PCR products and double-stranded DNA were digested to yield 19 bands, ranging from 100 bp – 10 kb, for molecular weight standards in agarose gel electrophoresis. The 500, 1,500, and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

Protocol  

1kb DNA Marker Non-Toxic - Green

$78.00

Description

Biocrede's non-toxic DNA markers contain a non-toxic and efficient alternative DNA visualizing to ethidium bromide, a potent carcinogen. The brightness is optimized for compatible and proper camera exposure in applications along with PCR products generated from Biocrede's 2X PCR MasterMixes as well as from any other SYBR Green-alike-based PCR amplification. To use, simply load 5 ul of the marker onto a plain gel and perform electrophoresis along with samples; Visualize the gel using an UV light source.

Package 500 ul/ 100 loads

Storage Store at 4°C for up to 3 months or -20°C for up to 24 months.

Protocol 

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1kb DNA Marker Plus

$48.00

Description

PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 14 bands, ranging from 250 pb – 25 kb, for molecular weight standards in agarose gel electrophoresis. The 1,000 and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

Protocol 

tab3