The use of TRIzol® in gene expression studies involving routine RNA extraction often poses bio-hazard and health concerns to scientist and researchers. RNAXtract Lysis Kit is the perfect solution to this problem.
RNAXtract Lysis Kit is Biocrede's unique and effective alternative method for RNA template extraction and preparation. RNA templates are extracted and processed directly from cultured cells for one-step or two-steps qRT-PCR in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template in either a one-step qRT-PCR or a two-step qRT-PCR system. In addition, samples can be directly processed in culture wells (96 and 384 wells) for easy handling and high throughput, thus minimizing transfer error and potential sample loss.
This product is only intended for quick RNA extraction from cultured cells for two-steps RT-PCR or two-steps qRT-PCR gene expression analysis.
This product is not suitable for typical RNA/cDNA pool preparation.
Biocrede's 100 bp DNA marker has been optimized for imaging alongside PCR products made from Biocrede Mastermixes or other SYBR Green-like PCR products.
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA. The bands range from 50 bp to 1.5 kb and show increased intensity at the 200 bp and 500 bp bands. The approximate mass of each band is provided for estimating the mass of DNA in comparison to DNA samples of similar size.
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Biocrede was established in June of 2012 (Ann Arbor, MI, USA) and is dedicated to transforming lives via innovative biomed-tech solutions that restore health, extend life, and reduce hospitalization length for all types of patients in the world, via comprehensive research, design, development, manufacturing and customer service in the eyes of our customers, employees, competitors, and healthcare communities. We are currently focusing on life sciences products to deliver custom viral vectors, pcr, diagnostics tools, and the research, development and commercialization of next generation medical solutions.
We believe our responsibility priority is to doctors, nurses, and patients, and thus we develop solutions, supply products, and provide services with high quality and ethics. We are responsible to our employees, and it is our desire to see every employee achieve their highest potential by striving to provide career counseling, appropriate training, job required education, and guidance with respect, dignity and merit recognition. We strive to play an integral role in to the society and the communities we live by creating value to help people globally have healtheir and more fulfilling lives, and make decisions that protect the environment and natural resources in the world. Our final responsibility is to our stockholders by making the business profitable.