Product Description
Biocrede's 2X PCR HotTaq MasterMix is a ready-to-use mixture of high quality hot start DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for amplification of DNA. HotTaq DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, Biocrede’s HotTaq DNA Polymerase offers improved yield of desired PCR products.
The 2X PCR HotTaq MasterMix with dye contains, in addition, an inert green dye blend and a stabilizer which allows for direct loading of the final products onto a gel for analysis. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.
Features and Benefits
- Saves preparation time by combining HotTaq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture.
- Reduces the risk of contamination by decreasing the number of pipetting steps.
- Provides consistent reaction performance and results.
Related Items
100bp DNA Marker
$48.00
Description
Biocrede's 100 bp DNA marker has been optimized for imaging alongside PCR products made from Biocrede Mastermixes or other SYBR Green-like PCR products.
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA. The bands range from 50 bp to 1.5 kb and show increased intensity at the 200 bp and 500 bp bands. The approximate mass of each band is provided for estimating the mass of DNA in comparison to DNA samples of similar size.
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