$48.00
Biocrede's 100 bp DNA marker has been optimized for imaging alongside PCR products made from Biocrede Mastermixes or other SYBR Green-like PCR products.
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA. The bands range from 50 bp to 1.5 kb and show increased intensity at the 200 bp and 500 bp bands. The approximate mass of each band is provided for estimating the mass of DNA in comparison to DNA samples of similar size.
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$78.00
Description
Biocrede's 100 bp Non-Toxic DNA Marker is a non-toxic and efficient alternative DNA visualizing agent to ethidium bromide, a potent carcinogen. The brightness is optimized for compatible and proper camera exposure in applications along with PCR products generated from Biocrede Master Mixes as well as from any other SYBR Green-alike-based PCR amplification. Simply load 5 ul of the marker onto a plain gel and perform electrophoresis along with samples; Visualize the gel using an UV light source.
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA.
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$48.00
Description
PCR products and double-stranded DNA were digested to yield 12 bands, ranging from 100 bp – 3 kb, for molecular weight standards in agarose gel electrophoresis. The 500 and 1,500 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
$48.00
Description
PCR products and double-stranded DNA were digested to yield 19 bands, ranging from 100 bp – 10 kb, for molecular weight standards in agarose gel electrophoresis. The 500, 1,500, and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
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$78.00
Description
Biocrede's non-toxic DNA markers contain a non-toxic and efficient alternative DNA visualizing to ethidium bromide, a potent carcinogen. The brightness is optimized for compatible and proper camera exposure in applications along with PCR products generated from Biocrede's 2X PCR MasterMixes as well as from any other SYBR Green-alike-based PCR amplification. To use, simply load 5 ul of the marker onto a plain gel and perform electrophoresis along with samples; Visualize the gel using an UV light source.
Package 500 ul/ 100 loads
Storage Store at 4°C for up to 3 months or -20°C for up to 24 months.
tab3
View full product details$48.00
Description
PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 14 bands, ranging from 250 pb – 25 kb, for molecular weight standards in agarose gel electrophoresis. The 1,000 and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.
tab3
View full product details$50.00
Biocrede's One-Step RT-PCR Kit contains all necessary reagents for both reverse transcription and PCR amplification to occur in a single reaction tube. Specifically, this One-Step RT-PCR kit contains RTase and UltraTaq DNA Polymerase in a convenient format for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-PCR buffer contains stabilizers and enhancers that optimize the two reactions in a “single step”. Together with a specially formulated RT-PCR buffer, this One-Step RT-PCR kit offers the end-users an efficient, easy to use and reliable alternative to conventional “two-step” sequential RT-PCR.
View full product details$160.00
Product Description
Removal of contaminating genomic DNA from your samples to reverse transcription of RNA in 30 minutes, with 5X All-In-One RT MasterMix (RTScript Genomic DNA Removal Kit included). Biocrede’s 5X All-in-One RT MasterMix (with RTScript Genomic DNA Removal Kit) offers a convenient and complete system to facilitate the complete elimination of contaminating genomic DNA in RNA preparations for rapid synthesis of high-quality cDNA for downstream applications.
Key Features
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
Application
$85.00
Product Description
From RNA template to cDNA in just 15 minutes completely hassle-free. With Biocrede’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of our MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience.
Key Features
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
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$30.00
Description
DNA Dye Non-Toxic products from Biocrede represent a new and safe class of nucleic acid stains for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose and polyacrylamide gels. The dyes are developed to replace toxic Ethidium Bromide (EB, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose and polyacrylamide gels. DNA Dye Non-Toxic products are non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.
Application
-Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.
Storage Store at 4°C
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$66.00
Description
Biocrede's non-toxic dyes represent a new and safe class of nucleic acid stains for the visualization of DNA in agarose and polyacrylamide gels. The dyes are developed to replace Ethidium Bromide, a potent mutagen, commonly used in gel electrophoresis for visualization of nucleic acids in agarose and polyacrylamide gels. This bundle comes with green, white, and red dyes that are all easy-to-use and non-toxic.
Application
-Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.
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$102.00
Product Description
DNase I is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.
Application
- Removes DNA from protein preparations and RNA samples
- Mediates nick translation
- Generates random fragments for dideoxy sequencing
- Degrade template DNA following in vitro transcription
- Mediate DNase I foot-printing
Storage 10 mM Tris-HCl (pH 7.6), 2 mM CaCl2, and 50% (v/v) Glycerol.
Handling Store all components at -20°C.
Notes One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.
This product is distributed for laboratory research only Caution: Not for diagnostic use
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$30.00
Product Description
The dNTP Mix is a ready-to-use aqueous solution containing dATP, dCTP, dGTP and dTTP, reducing the number of pippeting steps and the risk of errors.
Application
Ready to use in PCR, long-PCR, RT-PCR, cDNA synthesis, primer extention and DNA labeling.
Note: This product is distributed for laboratory research only.
View full product details$65.00
Product Description
The set consists of 100mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial. As the nucleotides are provided separately, the dNTP Set offers maximum flexibility in preparation of reaction mixes for different applications.
Application
Biocrede's dNTP Set is provided as 4 x 0.25ml 100mM aliquots.
View full product details$138.00
Product Description
Biocrede's Exactitaq is an industry-leading high-fidelity DNA polymerase. Biocrede's 2X PCR MasterMix provides all the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Exactitaq is the ideal choice for PCR that requires increased accuracy like cloning and sequencing, eliminating the need for downstream error correction steps. The use of Exactitaq DNA Polymerase for rare DNA templates is necessary as small amounts of DNA are especially prone to high mutant frequencies.
Application
• Whole genome sequencing
• Site-directed mutagenesis
• Blunt-end cloning
• Specific amplification of difficult templates (i.e. GC-Rich)
• Low copy PCR assays
Form 5 x 1.0 ml vials
Concentration 2x
Storage Store all components at -20 °C.
Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
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$138.00
Description
Biocrede's Exactitaq is an industry-leading high-fidelity DNA polymerase. Biocrede's 2X PCR MasterMix provides all the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Exactitaq is the ideal choice for PCR that requires increased accuracy like cloning and sequencing, eliminating the need for downstream error correction steps. The use of Exactitaq DNA Polymerase for rare DNA templates is necessary as small amounts of DNA are especially prone to high mutant frequencies. Precision MasterMix with dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region.
Application
• Whole genome sequencing
• Site-directed mutagenesis
• Blunt-end cloning
• Specific amplification of difficult templates (i.e. GC-Rich)
• Low copy PCR assays
Form 5 x 1.0 ml vials
Concentration 2x
Storage Store all components at -20 °C.
Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
$180.00
Description
Biocrede's Exactitaq is an industry-leading high-fidelity DNA polymerase. Significant time and effort can be saved by using high fidelity polymerases that eliminate the need for downstream error-correction steps. The use of Exactitaq DNA Polymerase for rare DNA templates is necessary as small amounts of DNA are especially prone to high mutant frequencies.
Application
• Whole genome sequencing
• Site-directed mutagenesis
• Blunt-end cloning
• Specific amplification of difficult templates (i.e. GC-Rich)
• Low copy PCR assays
Form Enzyme supplied with 5X buffer, MgSO4 and 5X GC Enhancer
Concentration 5U/µl
Storage Store all components at -20 °C.
Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
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$66.00
Product Description
Struggling to amplify long targets? Biocrede's Hawkeye Long Range DNA Polymerase will end your fight. Biocrede's Hawkeye Long Range DNA polymerase is capable of amplifying templates up to 20kb with minimal optimization. Coupled with superb performance, this enzyme’s enhanced extension rate drastically reduces the overall reaction time. Biocrede's Hawkeye Long Range DNA polymerase is the ideal choice for generating long PCR products with extreme sensitivity and processivity.
With Biocrede's Hawkeye Long-Range DNA Polymerase, you can expect:
Application
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$198.00
Description
Biocrede's Hemotaq DNA Polymerase 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. This enzyme is capable of performing PCR in reactions containing up to 20% (v/v) whole blood sample without a separate DNA purification step. As a result, the use of Biocrede's Hemotaq DNA Polymerase offers an overall reduction in contamination risk, experimental run time, and cost of genetic testing.
Applications
-Whole blood PCR
Form 5 x 1.0 ml vials
Concentration 2x
Storage Store all components at -20 °C.
Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
$66.00
Description
Biocrede's Hemotaq DNA Polymerase is capable of performing PCR in reactions containing up to 20% (v/v) whole blood sample without a separate DNA purification step. As a result, the use of Biocrede's Hemotaq DNA Polymerase offers an overall reduction in contamination risk, experimental run time, and cost of genetic testing.
Applications
-Whole blood PCR
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$168.00
Product Description
Biocrede's HiTaq DNA Polymerase is a novel DNA polymerase with strategically engineered mutations resulting in a robust, high-fidelity polymerase. HiTaq DNA polymerase has exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR.
Biocrede's HiTaq 2X PCR MasterMix is a ready-to-use mixture containing all the necessary reagents for highly successful amplification of DNA (high-fidelity DNA Polymerase, deoxynucleotides, reaction buffer and a sophisticated blend of additives in a 2X concentration). The HiTaq 2X PCR MasterMix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. HiTaq 2X PCR MasterMix is the most robust PCR MasterMix commercially available and will deliver an exceptional product yield with high fidelity.
HiTaq 2X PCR MasterMix with dye contains an inert green dye mixture and a stabilizer which allow for direct loading of the final PCR products onto an agarose gel for analysis. The green dye resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of the gel electrophoresis, and the yellow band indicates the migrating front of the gel.
Features and Benefits
$168.00
Product Description
Biocrede's HiTaq DNA Polymerase is a novel DNA polymerase with strategically engineered mutations resulting in a robust, high-fidelity polymerase. HiTaq DNA polymerase has exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR.
Biocrede's HiTaq 2X PCR MasterMix is a ready-to-use mixture containing all the necessary reagents for highly successful amplification of DNA (high-fidelity DNA Polymerase, deoxynucleotides, reaction buffer and a sophisticated blend of additives in a 2X concentration). The HiTaq 2X PCR MasterMix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. HiTaq 2X PCR MasterMix is the most robust PCR MasterMix commercially available and will deliver an exceptional product yield with high fidelity.
HiTaq 2X PCR MasterMix with dye contains an inert green dye mixture and a stabilizer which allow for direct loading of the final PCR products onto an agarose gel for analysis. The green dye resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of the gel electrophoresis, and the yellow band indicates the migrating front of the gel.
Features and Benefits
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$84.00
Product Description
Biocrede's HiTaq DNA Polymerase is a novel DNA polymerase with strategically engineered mutations resulting in a robust, high-fidelity polymerase. HiTaq DNA polymerase has exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR.
Biocrede's HiTaq 2X PCR MasterMix is a ready-to-use mixture containing all the necessary reagents for highly successful amplification of DNA (high-fidelity DNA Polymerase, deoxynucleotides, reaction buffer and a sophisticated blend of additives in a 2X concentration). The HiTaq 2X PCR MasterMix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. HiTaq 2X PCR MasterMix is the most robust PCR MasterMix commercially available and will deliver an exceptional product yield with high fidelity.
HiTaq 2X PCR MasterMix with dye contains an inert green dye mixture and a stabilizer which allow for direct loading of the final PCR products onto an agarose gel for analysis. The green dye resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of the gel electrophoresis, and the yellow band indicates the migrating front of the gel.
Features and Benefits
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$180.00
Product Description
Biocrede's 2X PCR HotTaq MasterMix is a ready-to-use mixture of high quality hot start DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for amplification of DNA. HotTaq DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, Biocrede’s HotTaq DNA Polymerase offers improved yield of desired PCR products.
The 2X PCR HotTaq MasterMix with dye contains, in addition, an inert green dye blend and a stabilizer which allows for direct loading of the final products onto a gel for analysis. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.
Features and Benefits
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$180.00
Product Description
Biocrede's 2X PCR HotTaq MasterMix is a ready-to-use mixture of high quality hot start DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentration. It contains all the necessary reagents for amplification of DNA. HotTaq DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, Biocrede’s HotTaq DNA Polymerase offers improved yield of desired PCR products.
The 2X PCR HotTaq MasterMix with dye contains, in addition, an inert green dye blend and a stabilizer which allows for direct loading of the final products onto a gel for analysis. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.
Features and Benefits
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$42.00
Product Description
HotTaq DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specifi c product formations that would otherwise competev for reagent availability. abm’s HotTaq DNA Polymerase offers higher specificity and improved yield of PCR products. PCR products amplifi ed up to 6 kb in length with HotTaq Taq DNA Polymerase contain a single base (A) 3’ overhang.
Features and Benefits
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$195.00
Product Description
One reaction tube for RT and PCR now offers unparalleled sensitivity and efficiency. With Biocrede’s One-Step RT-PCR Kit, you can streamline the procedure for reverse transcription and subsequent PCR in the same reaction tube, eliminating the need to change buffers and reducing the risk of contamination. This kit offers the end-user an efficient and easy alternative to the conventional “two-step” RT-PCR approach with extreme sensitivity, specificity and high product yield.
PCR-2010-dye buffer contains Cresol Red as an electrophoresis dye, with migration equivalent to 125 bp DNA fragment on an agarose gel.
Application
$144.00
Product Description
RNaseRid Ribonuclease Inhibitor specifically inhibits common ribonucleases (RNases), including RNase A, B and C, with high affinity. This enzyme is a useful additive in PCR or RT-PCR as it safe-guards RNA against potential RNase contamination without inhibiting polymerase activity. This robust version of RNase inhibitor has improved resistance to oxidation over the highly oxidation-sensitive human RNase inhibitor. RNaseRid Ribonuclease Inhibitor is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.
Product Applications
$54.00
Product Description
The use of TRIzol® in gene expression studies involving routine RNA extraction often poses bio-hazard and health concerns to scientist and researchers. RNAXtract Lysis Kit is the perfect solution to this problem.
RNAXtract Lysis Kit is Biocrede's unique and effective alternative method for RNA template extraction and preparation. RNA templates are extracted and processed directly from cultured cells for one-step or two-steps qRT-PCR in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template in either a one-step qRT-PCR or a two-step qRT-PCR system. In addition, samples can be directly processed in culture wells (96 and 384 wells) for easy handling and high throughput, thus minimizing transfer error and potential sample loss.
This product is only intended for quick RNA extraction from cultured cells for two-steps RT-PCR or two-steps qRT-PCR gene expression analysis.
This product is not suitable for typical RNA/cDNA pool preparation.
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$78.00
Product Description
Choose your priming methods with Biocrede’s cDNA Synthesis Kit. Biocrede's cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.
Key Features
• Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
• Robust cDNA synthesis from any RNA template
• High reproducibility and excellent yield
This kit employs Biocrede's RTScript Reverse Transcriptase
Application
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$50.00
Product Description
Convenient and time-saving reaction set-up with Biocrede’s cDNA Synthesis SuperMix. Biocrede’s cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, Biocrede’s cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease.
Key Features
• Streamlined protocol suitable for high-throughput applications
• Ease of use with a simple set-up
• Excellent cDNA yield
This SuperMix contains Biocrede’s RTScript Reverse Transcriptase.
Application
$42.00
Product Description
Biocrede’s RTScript Genomic Decontamination Kit offers a quick and easy method to eliminate genomic DNA (gDNA) contamination in RNA samples. The presence of genomic DNA in RNA preparations is often a significant problem for downstream applications. This contamination often leads to false-positive signals and misrepresentation of gene expression levels. This kit employs a brief reaction of your RNA sample with the enclosed Reaction Mix that will effectively removes gDNA without loss or degradation of RNA. The treated gDNA-free RNA is ready for any downstream application such as RT-PCR and qRT-PCR using Biocrede’s cDNA synthesis or RT-PCR reagents.
Key Features
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$60.00
Product Description
Convenient and time-saving reaction set-up with Biocrede’s RTScript Plus cDNA Synthesis SuperMix. Biocrede’s RTScript Plus cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, Biocrede’s RTScript Plus cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease.
Key Features
This SuperMix contains Biocrede’s RTScript Plus Reverse Transcriptase.
Application
$240.00
Product Description
Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with Biocrede’s RTScript Plus Reverse Transcriptase. Engineered to perform under high temperatures (45°C - 55°C), Biocrede’s RTScript Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, RTScript Plus Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.
Application
$66.00
Product Description
Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with Biocrede’s RTScript Plus Reverse Transcriptase. Engineered to perform under high temperatures (45°C - 55°C), Biocrede’s RTScript Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, RTScript Plus Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.
Application
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$60.00
Product Description
Choose your priming methods with Biocrede’s cDNA Synthesis Kit. Biocrede’s cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.
Key Features
This kit employs Biocrede’s RTScript Plus Reverse Transcriptase.
Application
$60.00
Product Description
Convenient and time-saving reaction set-up with Biocrede’s RTScript Two-Step RT-PCR Kit for complex RNA templates (i.e. secondary structures and high GC content). This highly efficient two-step RT-PCR kit consists of two main components: RTScript Plus cDNA Synthesis SuperMix and TaqPlus 2X PCR MasterMix with dye.
Biocrede’s cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, Biocrede’s cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. RTScript Plus is engineered to perform under high temperatures (45°C - 55°C), Biocrede’s RTScript Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length.
Biocrede’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, Biocrede offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. Biocrede’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
Key Features:
Application
$42.00
Product Description
Synthesize cDNA from only 0.1pg of RNA with Biocrede’s RTScript Reverse Transcriptase. Using strategic modifications, Biocrede’s RTScript Reverse Transcriptase lacks the intrinsic RNase H acitivity thereby drastically increases the yield and achievable synthesized cDNA length (up to 9kb). In addition, RTScript contains a fidelity-enhancing sub-unit which ensures superior accuracy in reverse transcription reaction(s).
Application$60.00
Product Description
Convenient and time-saving reaction set-up with Biocrede’s RTScript Two-Step RT-PCR Kit for complex RNA templates (i.e. secondary structures and high GC content). This highly efficient two-step RT-PCR kit consists of two main components: RTScript Plus cDNA Synthesis SuperMix and TaqPlus 2X PCR MasterMix with dye.
Biocrede’s cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, Biocrede’s cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. RTScript Plus is engineered to perform under high temperatures (45°C - 55°C), Biocrede’s RTScript Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length.
Biocrede’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, Biocrede offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. Biocrede’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
Key Features:
Application
$125.00
Product Description
It has been well established that many variables affect a particular PCR reaction: template structures, primer design, annealing temperature, concentration of Mg2+, etc. The Spectrum PCR Kit was developed to simplify the PCR optimization process. The system consists of multiple thermo-stable DNA polymerases pre-mixed with 12 optimized buffers in a 2X MasterMix format, saving a great amount of time in PCR set-up compared to the non-MasterMix PCR optimization format. To find the optimal conditions for your difficult PCR, all you need to do is to mix templates, primers, and H2O with the Spectrum PCR Kit 2X MasterMix
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$125.00
Product Description
It has been well established that many variables affect a particular PCR reaction: template structures, primer design, annealing temperature, concentration of Mg2+, etc. The Spectrum PCR Kit was developed to simplify the PCR optimization process. The system consists of multiple thermo-stable DNA polymerases pre-mixed with 12 optimized buffers in a 2X MasterMix format, saving a great amount of time in PCR set-up compared to the non-MasterMix PCR optimization format. To find the optimal conditions for your difficult PCR, all you need to do is to mix templates, primers, and H2O with the Spectrum PCR Kit 2X MasterMix
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$125.00
Product Description
It has been well established that many variables affect a particular PCR reaction: template structures, primer design, annealing temperature, concentration of Mg2+, etc. The Spectrum PCR Kit was developed to simplify the PCR optimization process. The system consists of multiple thermo-stable DNA polymerases pre-mixed with 12 optimized buffers in a 2X MasterMix format, saving a great amount of time in PCR set-up compared to the non-MasterMix PCR optimization format. To find the optimal conditions for your difficult PCR, all you need to do is to mix templates, primers, and H2O with the Spectrum PCR Kit 2X MasterMix
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$72.00
Product Description
Taq 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, Biocrede offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. Biocrede's MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
Application
$48.00
Product Description
Routine amplification in a cost-effective way? Biocrede’s Taq DNA Polymerase is the answer! Biocrede’s Taq DNA polymerase offers consistent results across a wide range of DNA templates with excellent yield and sensitivity. This conventional polymerase is suitable for most PCR applications where less emphasis is required on fidelity.
With Biocred's Taq DNA Polymerase, you can expect:
• Robust PCR performance with great reproducibility
• High sensitivity
Application
$96.00
Production Description
Streamline your PCR with Biocrede’s 2X PCR MasterMix. Biocrede’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, Biocrede offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. Biocrede’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.
The use of Taq DNA Polymerase in this MasterMix offers:
Application
tab2
Storage: Store all components at -20 °C.
Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains
Notes: This product is distributed for laboratory research only. dNTP sold seperately.
tab3
View full product details$42.00
Routine amplification in a cost-effective way? Biocrede’s Taq Plus DNA Polymerase is the answer. Biocrede’s Taq DNA polymerase offers consistent results across a wide range of DNA templates with excellent yield and sensitivity. This conventional polymerase is suitable for most PCR applications where less emphasis is required on fidelity.
With Biocrede's Taq DNA Polymerase, you can expect
Application
Storage: Store all components at -20 °C.
Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains
Notes: This product is distributed for laboratory research only. dNTP sold seperately.
tab3
View full product details$108.00
Product Description
With extension rate 6x faster than taq polymerase, Biocrede's TaqExpress 2X PCR MasterMix provides all the necessary ingredients for PCR in a premixed and optimized format that streamlines the PCR workflow. In addition to increased throughput, TaqExpress DNA polymerase also possesses moderate 3’-5’ proofreading activity, making this enzyme well suited for high-throughput PCR. Biocrede's TaqExpress is the solution to your fast and high-throughput PCR needs.
Applications
- Fast PCR
- High-throughput PCR
Polymerase | Target Length | Extension Speed (per min.) | Fidelity (vs. Taq) | Proofreading | MasterMix |
---|---|---|---|---|---|
Ultrataq | 15 kb | 3-4 kb | 50x | Yes | Yes |
Exactitaq | 6 kb | 1 kb | 60x | Yes | Yes |
Taq | 6 kb | 1 kb | 1x | No | Yes |
Hottaq | 6 kb | 1 kb | 1x | No | Yes |
Infinitaq | 11 kb | 4-6 kb | 10x | Yes | Yes |
Hawkeye | 20 kb | 3-4 kb | 1x | No | Yes |
Hemotaq | 1 kb | 1 kb | 1x | No | Yes |
Form 5 x 1.0 ml vials
Concentration 2x
Storage Store all components at -20 °C.
Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
View full product details
$108.00
Product Description
With extension rate 6x faster than taq polymerase Biocrede's TaqExpress 2X PCR MasterMix provides all the necessary ingredients for PCR in a premixed and optimized format that streamlines the PCR workflow. In addition to increased throughput, TaqFast DNA polymerase also possesses moderate 3’-5’ proofreading activity, making this enzyme well suited for high-throughput PCR. Biocrede's TaqExpress contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region.
Application
- Fast PCR
- High-throughput PCR
Polymerase | Target Length | Extension Speed (per min.) | Fidelity (vs. Taq) | Proofreading | MasterMix |
---|---|---|---|---|---|
Ultrataq | 15 kb | 3-4 kb | 50x | Yes | Yes |
Exactitaq | 6 kb | 1 kb | 60x | Yes | Yes |
Taq | 6 kb | 1 kb | 1x | No | Yes |
Hottaq | 6 kb | 1 kb | 1x | No | Yes |
Infinitaq | 11 kb | 4-6 kb | 10x | Yes | Yes |
Hawkeye | 20 kb | 3-4 kb | 1x | No | Yes |
Hemotaq | 1 kb | 1 kb | 1x | No | Yes |
Form 5 x 1.0 ml vials
Concentration 2x
Storage Store all components at -20 °C.
Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
tab3
View full product details$60.00
Product Description
With extension rate 6x faster than taq polymerase Biocrede's TaqExpress 2X PCR MasterMix provides all the necessary ingredients for PCR in a premixed and optimized format that streamlines the PCR workflow. In addition to improved processivity, TaqExpress DNA polymerase also possesses moderate 3’-5’ proofreading activity, making this enzyme well suited for high-throughput PCR. Biocrede's TaqExpress is the solution to your fast and high-throughput PCR needs.
Applications
- Fast PCR
Polymerase | Target Length | Extension Speed (per min.) | Fidelity (vs. Taq) | Proofreading | MasterMix |
---|---|---|---|---|---|
Ultrataq | 15 kb | 3-4 kb | 50x | Yes | Yes |
Exactitaq | 6 kb | 1 kb | 60x | Yes | Yes |
Taq | 6 kb | 1 kb | 1x | No | Yes |
Hottaq | 6 kb | 1 kb | 1x | No | Yes |
Infinitaq | 11 kb | 4-6 kb | 10x | Yes | Yes |
Hawkeye | 20 kb | 3-4 kb | 1x | No | Yes |
Hemotaq | 1 kb | 1 kb | 1x | No | Yes |
Concentration: 2x
Storage: Store all components at -20 °C.Notes
Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains
Notes: This product is distributed for laboratory research only.
tab3
View full product details$168.00
Product Description
Biocrede’s Ultrataq DNA Polymerase is a gold standard in next-generation DNA polymerase enzymes. Now you can streamline your PCR process with Biocrede’s Ultrataq 2x MasterMix. Biocrede’s 2x PCR MasterMix provides all of the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR process. Biocrede offers an array of MasterMixes to accommodate every PCR need. We also offer a line of MasterMixes that come with an inert blue dye and a stabilizer that allow for loading of the pcr products directly into an agarose gel.
Highlights
-Robust and high yield PCR across an array of templates.
-High processivity and shortened extension period.
-High fidelity, over 50x better than taq polymerase.
-Low cost per unit against comparable enzymes.
-Efficient amplification up to 16 kb.
-Robust amplification of AT and GC rich templates.
Application
-High-throughput PCR
-Amplification of long or difficult amplicons
-DNA cloning
-AT and GC rich templates
-RACE
-Template generation for NGS library construction
-Phylogenic Analysis
tab2
Picture 1: Ultrataq DNA Polymerase successfully amplifies genomic template up to 15.6 kb.
.
Polymerase | Target Length | Extension Speed (per min.) | Fidelity (vs. Taq) | Proofreading | MasterMix |
---|---|---|---|---|---|
Ultrataq | 15 kb | 3-4 kb | 50x | Yes | Yes |
Exactitaq | 6 kb | 1 kb | 60x | Yes | Yes |
Taq | 6 kb | 1 kb | 1x | No | Yes |
Hottaq | 6 kb | 1 kb | 1x | No | Yes |
Infinitaq | 11 kb | 4-6 kb | 10x | Yes | Yes |
Hawkeye | 20 kb | 3-4 kb | 1x | No | Yes |
Hemotaq | 1 kb | 1 kb | 1x | No | Yes |
Concentration: 2x
Storage: Store all components at -20 °C.Notes
Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains
Notes: This product is distributed for laboratory research only.
tab3
How can I optimize the reaction for multiplexing?
The polymerase and Mg concentrations need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.
Is Ultrataq DNA Polymerase compatible with Uracil?
Ultrataq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.
If no amplification products are obtained, what parameters should be considered first when troubleshooting?
First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.
If there is still no product the PCR conditions might be too stringent and should be changed as follows:
The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich.
If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem. Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.
Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?
No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on ultrataq PCR products, you can clean up the ultrataq PCR reaction first, then mix the cleaned-up ultrataq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the bestaq PCR products.
View full product details$168.00
Product Description
Biocrede’s Ultrataq DNA Polymerase is a gold standard in next-generation DNA polymerase enzymes. Now you can streamline your PCR with Biocrede’s Ultrataq 2x MasterMix with dye. Biocrede’s 2x PCR MasterMix with dye provides all of the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR process. This MasterMix comes with an inert blue dye and a stabilizer that allow for loading of the pcr products directly into an agarose gel, streamlining the process and reducing the risk of contamination.
Highlights
-Robust and high yield PCR across an array of templates.
-High processivity and shortened extension period.
-High fidelity, over 50x better than taq polymerase.
-Low cost per unit against comparable enzymes.
-Efficient amplification up to 16 kb.
-Robust amplification of AT and GC rich templates.
Application
-High-throughput PCR
-Amplification of long or difficult amplicons
-DNA cloning
-AT and GC rich templates
-RACE
-Template generation for NGS library construction
-Phylogenic Analysis
tab2
Picture 1: Ultrataq DNA Polymerase successfully amplifies genomic template up to 15.6 kb.
Polymerase | Target Length | Extension Speed (per min.) | Fidelity (vs. Taq) | Proofreading | MasterMix |
---|---|---|---|---|---|
Ultrataq | 15 kb | 3-4 kb | 50x | Yes | Yes |
Exactitaq | 6 kb | 1 kb | 60x | Yes | Yes |
Taq | 6 kb | 1 kb | 1x | No | Yes |
Hottaq | 6 kb | 1 kb | 1x | No | Yes |
Infinitaq | 11 kb | 4-6 kb | 10x | Yes | Yes |
Hawkeye | 20 kb | 3-4 kb | 1x | No | Yes |
Hemotaq | 1 kb | 1 kb | 1x | No | Yes |
Concentration: 5 U/μl
Storage: Store all components at -20 °C.Notes
Recommended products: UltraTaq master mix, UltraTaq master mix w/ dye, dNTP, Safe DNA stains
Notes: This product is distributed for laboratory research only.
tab3
How can I optimize the reaction for multiplexing?
The polymerase and Mg concentrations need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.
Is UltraTaq DNA Polymerase compatible with Uracil?
UltraTaq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.
If no amplification products are obtained, what parameters should be considered first when troubleshooting?
First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.
If there is still no product the PCR conditions might be too stringent and should be changed as follows:
The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich.
If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem. Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.
Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?
No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on UltraTaq PCR products, you can clean up the UltrTaq PCR reaction first, then mix the cleaned-up UltraTaq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the UltraTaq PCR products.
View full product details$84.00
Product Description
Looking to improve your routine PCR? UltraTaq DNA Polymerase is the new standard. With outstanding PCR yield, exceptional fidelity and high processivity, Biocrede’s UltraTaq DNA Polymerase is a versatile enzyme ideal for all PCR applications. With this superior enzyme, you can consolidate all PCR protocols and reactions into one efficient system.
With Biocrede’s UltraTaq DNA Polymerase, you can expect
tab3
How can I optimize the reaction for multiplexing?
The polymerase and Mg concentrations need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.
Is Ultrataq DNA Polymerase compatible with Uracil?
Ultrataq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.
If no amplification products are obtained, what parameters should be considered first when troubleshooting?
First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.
If there is still no product the PCR conditions might be too stringent and should be changed as follows:
The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich.
If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem. Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.
Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?
No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on ultrataq PCR products, you can clean up the UltraTaq PCR reaction first, then mix the cleaned-up ultrataq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the bestaq PCR products.
View full product details$84.00
Product Description
Looking to improve your routine PCR? UltraTaq DNA Polymerase is the new standard. With outstanding PCR yield, exceptional fidelity and high processivity, Biocrede’s UltraTaq DNA Polymerase is a versatile enzyme ideal for all PCR applications. With this superior enzyme, you can consolidate all PCR protocols and reactions into one efficient system.
With Biocrede’s UltraTaq DNA Polymerase, you can expect
Picture 1: Ultrataq DNA Polymerase successfully amplifies genomic template up to 15.6 kb.
Polymerase | Target Length | Extension Speed (per min.) | Fidelity (vs. Taq) | Proofreading | MasterMix |
---|---|---|---|---|---|
Ultrataq | 15 kb | 3-4 kb | 50x | Yes | Yes |
Exactitaq | 6 kb | 1 kb | 60x | Yes | Yes |
Taq | 6 kb | 1 kb | 1x | No | Yes |
Hottaq | 6 kb | 1 kb | 1x | No | Yes |
Infinitaq | 11 kb | 4-6 kb | 10x | Yes | Yes |
Hawkeye | 20 kb | 3-4 kb | 1x | No | Yes |
Hemotaq | 1 kb | 1 kb | 1x | No | Yes |
Concentration: 5 U/μl
Storage: Store all components at -20 °C.Notes
Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains
Is Ultrataq DNA Polymerase compatible with Uracil?Ultrataq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.
If no amplification products are obtained, what parameters should be considered first when troubleshooting?
First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.
If there is still no product the PCR conditions might be too stringent and should be changed as follows:
The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich.
If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem. Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.
Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?
No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on ultrataq PCR products, you can clean up the ultrataq PCR reaction first, then mix the cleaned-up ultrataq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the bestaq PCR products.
View full product details