100bp DNA Marker

$48.00

Biocrede's 100 bp DNA marker has been optimized for imaging alongside PCR products made from Biocrede Mastermixes or other SYBR Green-like PCR products. 

PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA. The bands range from 50 bp to 1.5 kb and show increased intensity at the 200 bp and 500 bp bands. The approximate mass of each band is provided for estimating the mass of DNA in comparison to DNA samples of similar size. 

Protocol  

100bp DNA Marker Non-Toxic - Green

$78.00

Description

Biocrede's 100 bp Non-Toxic DNA Marker is a non-toxic and efficient alternative DNA visualizing agent to ethidium bromide, a potent carcinogen. The brightness is optimized for compatible and proper camera exposure in applications along with PCR products generated from Biocrede Master Mixes as well as from any other SYBR Green-alike-based PCR amplification. Simply load 5 ul of the marker onto a plain gel and perform electrophoresis along with samples; Visualize the gel using an UV light source. 

PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH 8.0) and 1mM EDTA.

Protocol  

100bp DNA Marker Plus

$48.00

Description

PCR products and double-stranded DNA were digested  to yield 12 bands, ranging from 100 bp – 3 kb, for molecular weight standards in agarose gel electrophoresis. The 500 and 1,500 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

Protocol  

1kb DNA Marker

$48.00

Description

PCR products and double-stranded DNA were digested to yield 19 bands, ranging from 100 bp – 10 kb, for molecular weight standards in agarose gel electrophoresis. The 500, 1,500, and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

Protocol  

1kb DNA Marker Non-Toxic - Green

$78.00

Description

Biocrede's non-toxic DNA markers contain a non-toxic and efficient alternative DNA visualizing to ethidium bromide, a potent carcinogen. The brightness is optimized for compatible and proper camera exposure in applications along with PCR products generated from Biocrede's 2X PCR MasterMixes as well as from any other SYBR Green-alike-based PCR amplification. To use, simply load 5 ul of the marker onto a plain gel and perform electrophoresis along with samples; Visualize the gel using an UV light source.

Package 500 ul/ 100 loads

Storage Store at 4°C for up to 3 months or -20°C for up to 24 months.

Protocol 

tab3

1kb DNA Marker Plus

$48.00

Description

PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 14 bands, ranging from 250 pb – 25 kb, for molecular weight standards in agarose gel electrophoresis. The 1,000 and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

Protocol 

tab3

2X One-Step RT-PCR Buffer

$50.00

Biocrede's One-Step RT-PCR Kit contains all necessary reagents for both reverse transcription and PCR amplification to occur in a single reaction tube. Specifically, this One-Step RT-PCR kit contains RTase and UltraTaq DNA Polymerase in a convenient format for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-PCR buffer contains stabilizers and enhancers that optimize the two reactions in a “single step”. Together with a specially formulated RT-PCR buffer, this One-Step RT-PCR kit offers the end-users an efficient, easy to use and reliable alternative to conventional “two-step” sequential RT-PCR.

5X All-In-One MasterMix (DNA decontamination kit)

$160.00

Product Description

Removal of contaminating genomic DNA from your samples to reverse transcription of RNA in 30 minutes, with 5X All-In-One RT MasterMix (RTScript Genomic DNA Removal Kit included). Biocrede’s 5X All-in-One RT MasterMix (with RTScript Genomic DNA Removal Kit) offers a convenient and complete system to facilitate the complete elimination of contaminating genomic DNA in RNA preparations for rapid synthesis of high-quality cDNA for downstream applications.

Key Features

• Efficient ds and ssDNA digestion in under 10 minutes
• Maximal RNA protection – No heating or organic extraction steps required
• Fewer handling errors, with only 1 liquid transfer step for reverse transcription
• Streamlined protocol suitable for high-throughput applications
• Simple set-up for any RNA template
• High reproducibility and excellent cDNA yield

Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.

Application

• Generation of templates for use in RT-PCR and qRT-PCR
• cDNA synthesis from ssRNA
• cDNA library construction
• Generation of probes for hybridization
• DNA primer extension

5X All-In-One RTScript MasterMix

$85.00

Product Description

From RNA template to cDNA in just 15 minutes completely hassle-free. With Biocrede’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of our MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience.

Key Features

• Reduction in handling errors with only 1 liquid transfer step
• Streamlined protocol suitable for high-throughput applications
• Simple set-up for any RNA template
• High reproducibility and excellent yield

Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.

Protocol  

DNA Dye Non-Toxic

$30.00

Description

DNA Dye Non-Toxic products from Biocrede represent a new and safe class of nucleic acid stains for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose and polyacrylamide gels. The dyes are developed to replace toxic Ethidium Bromide (EB, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose and polyacrylamide gels. DNA Dye Non-Toxic products are non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

Application

-Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.

 Protocol  

Storage Store at 4°C

DNA Dye Non-Toxic- Bundle (W, G, R)

$66.00

Description

Biocrede's non-toxic dyes represent a new and safe class of nucleic acid stains for the visualization of DNA in agarose and polyacrylamide gels. The dyes are developed to replace Ethidium Bromide, a potent mutagen, commonly used in gel electrophoresis for visualization of nucleic acids in agarose and polyacrylamide gels. This bundle comes with green, white, and red dyes that are all easy-to-use and non-toxic.

Application

-Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.

Protocol  

DNase I

$102.00

Product Description

DNase I is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.

Application

- Removes DNA from protein preparations and RNA samples
- Mediates nick translation
- Generates random fragments for dideoxy sequencing
- Degrade template DNA following in vitro transcription
- Mediate DNase I foot-printing

Storage 10 mM Tris-HCl (pH 7.6), 2 mM CaCl₂, and 50% (v/v) Glycerol.

Handling Store all components at -20°C.

Protocol   

Notes One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.

This product is distributed for laboratory research only Caution: Not for diagnostic use

dNTP Mix

$30.00

Product Description

The dNTP Mix is a ready-to-use aqueous solution containing dATP, dCTP, dGTP and dTTP, reducing the number of pippeting steps and the risk of errors.

Application

Ready to use in PCR, long-PCR, RT-PCR, cDNA synthesis, primer extention and DNA labeling.

Note: This product is distributed for laboratory research only.

Protocol  

dNTP Set

$65.00

Product Description

The set consists of 100mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial. As the nucleotides are provided separately, the dNTP Set offers maximum flexibility in preparation of reaction mixes for different applications.

Application

• PCR
• Long PCR
• RT-PCR
• cDNA Synthesis
• Primer Extension
• DNA sequencing
• DNA labeling

Biocrede's dNTP Set is provided as 4 x 0.25ml 100mM aliquots.

Protocol 

ExactiTaq 2X PCR Master Mix

$138.00

Product Description

Biocrede's Exactitaq is an industry-leading high-fidelity DNA polymerase.  Biocrede's 2X PCR MasterMix provides all the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Exactitaq is the ideal choice for PCR that requires increased accuracy like cloning and sequencing, eliminating the need for downstream error correction steps. The use of Exactitaq DNA Polymerase for rare DNA templates is necessary as small amounts of DNA are especially prone to high mutant frequencies. 

Application

• Whole genome sequencing
• Site-directed mutagenesis
• Blunt-end cloning
• Specific amplification of difficult templates (i.e. GC-Rich)
• Low copy PCR assays

Form 5 x 1.0 ml vials

Concentration 2x

Storage Store all components at -20 °C.

Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

Protocol  

ExactiTaq 2X PCR Master Mix - Dye

$138.00

Description

Biocrede's Exactitaq is an industry-leading high-fidelity DNA polymerase.  Biocrede's 2X PCR MasterMix provides all the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Exactitaq is the ideal choice for PCR that requires increased accuracy like cloning and sequencing, eliminating the need for downstream error correction steps. The use of Exactitaq DNA Polymerase for rare DNA templates is necessary as small amounts of DNA are especially prone to high mutant frequencies. Precision MasterMix with dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region.

Application

• Whole genome sequencing
• Site-directed mutagenesis
• Blunt-end cloning
• Specific amplification of difficult templates (i.e. GC-Rich)
• Low copy PCR assays

Form 5 x 1.0 ml vials

Concentration 2x

Storage Store all components at -20 °C.

Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

Protocol 

ExactiTaq DNA Polymerase

$180.00

Description

Biocrede's Exactitaq is an industry-leading high-fidelity DNA polymerase. Significant time and effort can be saved by using high fidelity polymerases that eliminate the need for downstream error-correction steps. The use of Exactitaq DNA Polymerase for rare DNA templates is necessary as small amounts of DNA are especially prone to high mutant frequencies. 

Application

• Whole genome sequencing
• Site-directed mutagenesis
• Blunt-end cloning
• Specific amplification of difficult templates (i.e. GC-Rich)
• Low copy PCR assays

Form Enzyme supplied with 5X buffer, MgSO₄ and 5X GC Enhancer

Concentration 5U/µl

Storage Store all components at -20 °C.

Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

Protocol   

Hawkeye Long Range DNA Polymerase

$66.00

Product Description

Struggling to amplify long targets? Biocrede's Hawkeye Long Range DNA Polymerase will end your fight. Biocrede's Hawkeye Long Range DNA polymerase is capable of amplifying templates up to 20kb with minimal optimization. Coupled with superb performance, this enzyme’s enhanced extension rate drastically reduces the overall reaction time. Biocrede's Hawkeye Long Range DNA polymerase is the ideal choice for generating long PCR products with extreme sensitivity and processivity.

With Biocrede's Hawkeye Long-Range DNA Polymerase, you can expect:

• High-speed PCR without compromising accuracy
• High-processivity to reduce reaction time by up to 70%
• Efficient amplification of DNA templates up to 20kb

Application

• Long range PCR
• Robust amplification of AT- and GC-Rich sequences

 Protocol   

HemoTaq 2X PCR Master Mix

$198.00

Description

Biocrede's Hemotaq DNA Polymerase 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. This enzyme is capable of performing PCR in reactions containing up to 20% (v/v) whole blood sample without a separate DNA purification step. As a result, the use of Biocrede's Hemotaq DNA Polymerase offers an overall reduction in contamination risk, experimental run time, and cost of genetic testing.

Applications

-Whole blood PCR

Form 5 x 1.0 ml vials

Concentration 2x

Storage Store all components at -20 °C.

Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

Protocol  

HemoTaq DNA Polymerase

$66.00

Description

Biocrede's Hemotaq DNA Polymerase is capable of performing PCR in reactions containing up to 20% (v/v) whole blood sample without a separate DNA purification step. As a result, the use of Biocrede's Hemotaq DNA Polymerase offers an overall reduction in contamination risk, experimental run time, and cost of genetic testing.

Applications

-Whole blood PCR

Protocol  

HiTaq 2X PCR Master Mix

$168.00

Product Description

Biocrede's HiTaq DNA Polymerase is a novel DNA polymerase with strategically engineered mutations resulting in a robust, high-fidelity polymerase. HiTaq DNA polymerase has exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR.

 Biocrede's HiTaq 2X PCR MasterMix is a ready-to-use mixture containing all the necessary reagents for highly successful amplification of DNA (high-fidelity DNA Polymerase, deoxynucleotides, reaction buffer and a sophisticated blend of additives in a 2X concentration). The HiTaq 2X PCR MasterMix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. HiTaq 2X PCR MasterMix is the most robust PCR MasterMix commercially available and will deliver an exceptional product yield with high fidelity.

 HiTaq 2X PCR MasterMix with dye contains an inert green dye mixture and a stabilizer which allow for direct loading of the final PCR products onto an agarose gel for analysis. The green dye resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of the gel electrophoresis, and the yellow band indicates the migrating front of the gel.

Features and Benefits

• Saves preparation time by combining DNA Polymerase, dNTPs and reaction buffer in a ready-to-use 2X mixture.
• Robust PCR performance with high yield and superb fidelity.
• Compatible with plant DNA templates.
• Reduces the risk of contamination by decreasing the number of pipetting steps.
• Provides consistent performance and results.

Protocol  

HiTaq 2X PCR Master Mix - Dye

$168.00

Product Description

Biocrede's HiTaq DNA Polymerase is a novel DNA polymerase with strategically engineered mutations resulting in a robust, high-fidelity polymerase. HiTaq DNA polymerase has exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR.

 Biocrede's HiTaq 2X PCR MasterMix is a ready-to-use mixture containing all the necessary reagents for highly successful amplification of DNA (high-fidelity DNA Polymerase, deoxynucleotides, reaction buffer and a sophisticated blend of additives in a 2X concentration). The HiTaq 2X PCR MasterMix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. HiTaq 2X PCR MasterMix is the most robust PCR MasterMix commercially available and will deliver an exceptional product yield with high fidelity.

 HiTaq 2X PCR MasterMix with dye contains an inert green dye mixture and a stabilizer which allow for direct loading of the final PCR products onto an agarose gel for analysis. The green dye resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of the gel electrophoresis, and the yellow band indicates the migrating front of the gel.

Features and Benefits

• Saves preparation time by combining DNA Polymerase, dNTPs and reaction buffer in a ready-to-use 2X mixture.
• Robust PCR performance with high yield and superb fidelity.
• Compatible with plant DNA templates.
• Reduces the risk of contamination by decreasing the number of pipetting steps.
• Provides consistent performance and results.

Protocol  

HiTaq DNA Polymerase

$84.00

Product Description

Biocrede's HiTaq DNA Polymerase is a novel DNA polymerase with strategically engineered mutations resulting in a robust, high-fidelity polymerase. HiTaq DNA polymerase has exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR.

 Biocrede's HiTaq 2X PCR MasterMix is a ready-to-use mixture containing all the necessary reagents for highly successful amplification of DNA (high-fidelity DNA Polymerase, deoxynucleotides, reaction buffer and a sophisticated blend of additives in a 2X concentration). The HiTaq 2X PCR MasterMix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. HiTaq 2X PCR MasterMix is the most robust PCR MasterMix commercially available and will deliver an exceptional product yield with high fidelity.

 HiTaq 2X PCR MasterMix with dye contains an inert green dye mixture and a stabilizer which allow for direct loading of the final PCR products onto an agarose gel for analysis. The green dye resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of the gel electrophoresis, and the yellow band indicates the migrating front of the gel.

Features and Benefits

• Saves preparation time by combining DNA Polymerase, dNTPs and reaction buffer in a ready-to-use 2X mixture.
• Robust PCR performance with high yield and superb fidelity.
• Compatible with plant DNA templates.
• Reduces the risk of contamination by decreasing the number of pipetting steps.
• Provides consistent performance and results.

Protocol  

HotTaq 2X PCR Master Mix

$180.00

Product Description

The 2X PCR HotTaq MasterMix with dye contains, in addition, an inert green dye blend and a stabilizer which allows for direct loading of the final products onto a gel for analysis. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.

Features and Benefits

• Saves preparation time by combining HotTaq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture.
• Reduces the risk of contamination by decreasing the number of pipetting steps.
• Provides consistent reaction performance and results.

Protocol  

HotTaq 2X PCR Master Mix- Dye

$180.00

Product Description

The 2X PCR HotTaq MasterMix with dye contains, in addition, an inert green dye blend and a stabilizer which allows for direct loading of the final products onto a gel for analysis. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.

Features and Benefits

• Saves preparation time by combining HotTaq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture.
• Reduces the risk of contamination by decreasing the number of pipetting steps.
• Provides consistent reaction performance and results.

Protocol 

HotTaq DNA Polymerase

$42.00

Product Description

HotTaq DNA Polymerase contains a proprietary antibody that blocks polymerase activity at low temperatures. During the initial denaturation step at 94°C, the antibody dissociates from Taq DNA polymerase and restores enzyme activity. This feature significantly reduces non-specifi c product formations that would otherwise competev for reagent availability. abm’s HotTaq DNA Polymerase offers higher specificity and improved yield of PCR products. PCR products amplifi ed up to 6 kb in length with HotTaq Taq DNA Polymerase contain a single base (A) 3’ overhang.

Features and Benefits

• Saves preparation time by combining HotTaq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture.
• Reduces the risk of contamination by decreasing the number of pipetting steps.
• Provides consistent reaction performance and results.

Protocol   

One-Step RT-PCR Kit

$195.00

Product Description

One reaction tube for RT and PCR now offers unparalleled sensitivity and efficiency. With Biocrede’s  One-Step RT-PCR Kit, you can streamline the procedure for reverse transcription and subsequent PCR in the same reaction tube, eliminating the need to change buffers and reducing the risk of contamination. This kit offers the end-user an efficient and easy alternative to the conventional “two-step” RT-PCR approach with extreme sensitivity, specificity and high product yield.

PCR-2010-dye buffer contains Cresol Red as an electrophoresis dye, with migration equivalent to 125 bp DNA fragment on an agarose gel.

Application

• Gene-expression analysis
• Transcription analysis
• Gene cloning
• Multiplex RT-PCR
• High throughput applications
• Virus detection and quantification

Protocol  

RNaseRID Ribonuclease Inhibitor

$144.00

Product Description

RNaseRid Ribonuclease Inhibitor specifically inhibits common ribonucleases (RNases), including RNase A, B and C, with high affinity. This enzyme is a useful additive in PCR or RT-PCR as it safe-guards RNA against potential RNase contamination without inhibiting polymerase activity. This robust version of RNase inhibitor has improved resistance to oxidation over the highly oxidation-sensitive human RNase inhibitor. RNaseRid Ribonuclease Inhibitor is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

Product Applications

• Protection of RNA during:

• Preparation of RNase-free antibodies
• Contamination-sensitive experiments

RNAXtract Cell Lysis Kit

$54.00

Product Description

The use of TRIzol® in gene expression studies involving routine RNA extraction often poses bio-hazard and health concerns to scientist and researchers. RNAXtract Lysis Kit is the perfect solution to this problem.
RNAXtract Lysis Kit is Biocrede's unique and effective alternative method for RNA template extraction and preparation. RNA templates are extracted and processed directly from cultured cells for one-step or two-steps qRT-PCR in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template in either a one-step qRT-PCR or a two-step qRT-PCR system. In addition, samples can be directly processed in culture wells (96 and 384 wells) for easy handling and high throughput, thus minimizing transfer error and potential sample loss.

This product is only intended for quick RNA extraction from cultured cells for two-steps RT-PCR or two-steps qRT-PCR gene expression analysis.

This product is not suitable for typical RNA/cDNA pool preparation.

RTScript cDNA Synthesis Kit

$78.00

Product Description

Choose your priming methods with Biocrede’s cDNA Synthesis Kit. Biocrede's cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.

Key Features
• Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
• Robust cDNA synthesis from any RNA template
• High reproducibility and excellent yield

This kit employs Biocrede's RTScript Reverse Transcriptase

Application

• Synthesizing cDNA from ssRNA
• DNA primer extension
• Sequencing dsDNA
• Constructing cDNA library
• Producing template for use in RT-PCR
• Labelling 3'-end of duplex DNA via end-filling reactions
• Generating probes for hybridization

RTScript cDNA Synthesis SuperMix Kit

$50.00

Product Description

Convenient and time-saving reaction set-up with Biocrede’s cDNA Synthesis SuperMix. Biocrede’s cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, Biocrede’s cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease.

Key Features
• Streamlined protocol suitable for high-throughput applications
• Ease of use with a simple set-up
• Excellent cDNA yield

This SuperMix contains Biocrede’s  RTScript Reverse Transcriptase.

Application

• Generation of templates for use in RT-PCR and qRT-PCR
  • cDNA synthesis from ssRNA
  • cDNA library construction
  • Generation of probes for hybridization
  • DNA primer extension

RTScript Genomic Decontamination Kit

$42.00

Product Description

 Biocrede’s RTScript Genomic Decontamination  Kit offers a quick and easy method to eliminate genomic DNA (gDNA) contamination in RNA samples. The presence of genomic DNA in RNA preparations is often a significant problem for downstream applications. This contamination often leads to false-positive signals and misrepresentation of gene expression levels. This kit employs a brief reaction of your RNA sample with the enclosed Reaction Mix that will effectively removes gDNA without loss or degradation of RNA. The treated gDNA-free RNA is ready for any downstream application such as RT-PCR and qRT-PCR using Biocrede’s cDNA synthesis or RT-PCR reagents.

Key Features

• Fast and easy procedure completed within 10 minutes
• Complete and effective genomic DNA removal without compromising RNA quality
• No heat inactivation required
• Compatible with various downstream applications

 Protocol  

RTScript Plus cDNA Synthesis SuperMix

$60.00

Product Description

Convenient and time-saving reaction set-up with Biocrede’s RTScript Plus cDNA Synthesis SuperMix. Biocrede’s RTScript Plus cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, Biocrede’s RTScript Plus cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease.

Key Features

• Streamlined protocol suitable for high-throughput applications
• Ease of use with a simple set-up
• Excellent cDNA yield

This SuperMix contains Biocrede’s RTScript Plus Reverse Transcriptase.

Application       

• Generation of templates for use in RT-PCR and qRT-PCR
• cDNA synthesis from ssRNA
• cDNA library construction
• Generation of probes for hybridization
• DNA primer extension

RTScript Plus Reverse Transcriptase

$240.00

Product Description

Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with Biocrede’s RTScript Plus Reverse Transcriptase. Engineered to perform under high temperatures (45°C - 55°C), Biocrede’s RTScript Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, RTScript Plus Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.

Application

• Synthesizing cDNA from a ssRNA
• DNA primer extension
• Sequencing dsDNA
• Constructing cDNA library
• Constructing libraries for serial analysis for gene expression (SAGE)
• Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE)
• Producing template for use in RT-PCR or real-time RT-PCR
• Labelling 3'-end of duplex DNA via end-filling reactions
• Generating probes for hybridization

RTScript Plus Reverse Transcriptase

$66.00

 Product Description

Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with Biocrede’s RTScript Plus Reverse Transcriptase. Engineered to perform under high temperatures (45°C - 55°C), Biocrede’s RTScript Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, RTScript Plus Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.

Application

• Synthesizing cDNA from a ssRNA
• DNA primer extension
• Sequencing dsDNA
• Constructing cDNA library
• Constructing libraries for serial analysis for gene expression (SAGE)
• Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE)
• Producing template for use in RT-PCR or real-time RT-PCR
• Labelling 3'-end of duplex DNA via end-filling reactions
• Generating probes for hybridization

RTScript Plus Synthesis Kit

$60.00

Product Description

Choose your priming methods with Biocrede’s cDNA Synthesis Kit. Biocrede’s cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.

Key Features

• Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
• Robust cDNA synthesis from any RNA template
• High reproducibility and excellent yield

This kit employs Biocrede’s RTScript Plus Reverse Transcriptase.

Application

• Synthesizing cDNA from ssRNA
• DNA primer extension
• Sequencing dsDNA
• Constructing cDNA library
• Producing template for use in RT-PCR
• Labelling 3'-end of duplex DNA via end-filling reactions
• Generating probes for hybridization

RTScript Plus Two-Step RT-PCR

$60.00

RTScript Reverse Transcriptase

$42.00

Product Description

Synthesize cDNA from only 0.1pg of RNA with Biocrede’s RTScript Reverse Transcriptase. Using strategic modifications, Biocrede’s RTScript Reverse Transcriptase lacks the intrinsic RNase H acitivity thereby drastically increases the yield and achievable synthesized cDNA length (up to 9kb). In addition, RTScript contains a fidelity-enhancing sub-unit which ensures superior accuracy in reverse transcription reaction(s).

• Synthesizing cDNA from a ssRNA
• DNA primer extension
• Sequencing dsDNA
• Constructing cDNA library
• Constructing libraries for serial analysis for gene expression (SAGE)
• Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE)
• Producing template for use in RT-PCR or real-time RT-PCR
• Labelling 3'-end of duplex DNA via end-filling reactions
• Generating probes for hybridization.

 Protocol 

RTScript Two-Step RT-PCR

$60.00

Spectrum PCR Mix 06

$125.00

Product Description

It has been well established that many variables affect a particular PCR reaction: template structures, primer design, annealing temperature, concentration of Mg²⁺, etc. The Spectrum PCR Kit was developed to simplify the PCR optimization process. The system consists of multiple thermo-stable DNA polymerases pre-mixed with 12 optimized buffers in a 2X MasterMix format, saving a great amount of time in PCR set-up compared to the non-MasterMix PCR optimization format. To find the optimal conditions for your difficult PCR, all you need to do is to mix templates, primers, and H₂O with the Spectrum PCR Kit 2X MasterMix

Spectrum PCR Mix 09

$125.00

Product Description

It has been well established that many variables affect a particular PCR reaction: template structures, primer design, annealing temperature, concentration of Mg²⁺, etc. The Spectrum PCR Kit was developed to simplify the PCR optimization process. The system consists of multiple thermo-stable DNA polymerases pre-mixed with 12 optimized buffers in a 2X MasterMix format, saving a great amount of time in PCR set-up compared to the non-MasterMix PCR optimization format. To find the optimal conditions for your difficult PCR, all you need to do is to mix templates, primers, and H₂O with the Spectrum PCR Kit 2X MasterMix

Spectrum PCR Optimization Kit (Mix 1 to 12)

$125.00

 Product Description

It has been well established that many variables affect a particular PCR reaction: template structures, primer design, annealing temperature, concentration of Mg²⁺, etc. The Spectrum PCR Kit was developed to simplify the PCR optimization process. The system consists of multiple thermo-stable DNA polymerases pre-mixed with 12 optimized buffers in a 2X MasterMix format, saving a great amount of time in PCR set-up compared to the non-MasterMix PCR optimization format. To find the optimal conditions for your difficult PCR, all you need to do is to mix templates, primers, and H₂O with the Spectrum PCR Kit 2X MasterMix

Taq 2X PCR MasterMix

$72.00

Product Description

Taq 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, Biocrede offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. Biocrede's MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.

Application

• Routine PCR amplification of DNA templates up to 6 kb
• Suitable for a wide range of PCR assays
• TA cloning

 Protocol  

Taq DNA Polymerase

$48.00

Product Description

Routine amplification in a cost-effective way? Biocrede’s Taq DNA Polymerase is the answer! Biocrede’s Taq DNA polymerase offers consistent results across a wide range of DNA templates with excellent yield and sensitivity. This conventional polymerase is suitable for most PCR applications where less emphasis is required on fidelity.

With Biocred's Taq DNA Polymerase, you can expect:
• Robust PCR performance with great reproducibility
• High sensitivity

Application

• Routine PCR amplification of DNA templates up to 6 kb
• Suitable for a wide range of PCR assays
• TA cloning

Protocol  

Taq Plus 2X PCR Master Mix

$96.00

Production Description

Streamline your PCR with Biocrede’s 2X PCR MasterMix. Biocrede’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, Biocrede offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. Biocrede’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.

 The use of Taq DNA Polymerase in this MasterMix offers:

• Robust PCR performance with great reproducibility
• High sensitivity

Application

• Routine PCR amplification of DNA templates up to 6 kb
• Suitable for a wide range of PCR assays
• TA cloning

Protocol  

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Storage: Store all components at -20 °C.

Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains

Notes: This product is distributed for laboratory research only. dNTP sold seperately.

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Taq Plus DNA Polymerase

$42.00

Routine amplification in a cost-effective way? Biocrede’s Taq Plus DNA Polymerase is the answer. Biocrede’s Taq DNA polymerase offers consistent results across a wide range of DNA templates with excellent yield and sensitivity. This conventional polymerase is suitable for most PCR applications where less emphasis is required on fidelity.

With Biocrede's Taq DNA Polymerase, you can expect

• Robust PCR performance with great reproducibility
• High sensitivity

Application

• Routine PCR amplification of DNA templates up to 6 kb
• Suitable for a wide range of PCR assays
• TA cloning

Storage: Store all components at -20 °C.

Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains

Notes: This product is distributed for laboratory research only. dNTP sold seperately.

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TaqExpress 2X PCR Master Mix

$108.00

Product Description

With extension rate 6x faster than taq polymerase, Biocrede's TaqExpress 2X PCR MasterMix provides all the necessary ingredients for PCR in a premixed and optimized format that streamlines the PCR workflow.  In addition to increased throughput, TaqExpress DNA polymerase also possesses moderate 3’-5’ proofreading activity, making this enzyme well suited for high-throughput PCR.  Biocrede's TaqExpress is the solution to your fast and high-throughput PCR needs.

Applications

- Fast PCR

- High-throughput PCR

Form 5 x 1.0 ml vials

Concentration 2x

Storage Store all components at -20 °C.

Protocol  

Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

TaqExpress 2X PCR Master Mix- Dye

$108.00

Product Description

With extension rate 6x faster than taq polymerase Biocrede's TaqExpress 2X PCR MasterMix provides all the necessary ingredients for PCR in a premixed and optimized format that streamlines the PCR workflow.  In addition to increased throughput, TaqFast DNA polymerase also possesses moderate 3’-5’ proofreading activity, making this enzyme well suited for high-throughput PCR.  Biocrede's TaqExpress contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region.

Application

- Fast PCR
- High-throughput PCR

Form 5 x 1.0 ml vials

Concentration 2x

Storage Store all components at -20 °C.

Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

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TaqExpress DNA Polymerase

$60.00

Product Description

With extension rate 6x faster than taq polymerase Biocrede's TaqExpress 2X PCR MasterMix provides all the necessary ingredients for PCR in a premixed and optimized format that streamlines the PCR workflow.  In addition to improved processivity, TaqExpress DNA polymerase also possesses moderate 3’-5’ proofreading activity, making this enzyme well suited for high-throughput PCR.  Biocrede's TaqExpress is the solution to your fast and high-throughput PCR needs.

Applications

Protocol  

Concentration: 2x

Storage: Store all components at -20 °C.Notes

Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains

Notes: This product is distributed for laboratory research only.

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UltraTaq 2X PCR Master Mix

$168.00

Product Description

Biocrede’s Ultrataq DNA Polymerase is a gold standard in next-generation DNA polymerase enzymes. Now you can streamline your PCR process with Biocrede’s Ultrataq 2x MasterMix. Biocrede’s 2x PCR MasterMix provides all of the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR process. Biocrede offers an array of MasterMixes to accommodate every PCR need. We also offer a line of MasterMixes that come with an inert blue dye and a stabilizer that allow for loading of the pcr products directly into an agarose gel.

Highlights
-Robust and high yield PCR across an array of templates.
-High processivity and shortened extension period.
-High fidelity, over 50x better than taq polymerase.
-Low cost per unit against comparable enzymes.
-Efficient amplification up to 16 kb.
-Robust amplification of AT and GC rich templates.

Application
-High-throughput PCR
-Amplification of long or difficult amplicons
-DNA cloning
-AT and GC rich templates
-RACE                            
-Template generation for NGS library construction
-Phylogenic Analysis

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Picture 1: Ultrataq DNA Polymerase successfully amplifies genomic template up to 15.6 kb.

. 

Protocol  

Concentration: 2x

Storage: Store all components at -20 °C.Notes

Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains

Notes: This product is distributed for laboratory research only.

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How can I optimize the reaction for multiplexing?

The polymerase and Mg concentrations need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.

Is Ultrataq DNA Polymerase compatible with Uracil?

Ultrataq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.

If no amplification products are obtained, what parameters should be considered first when troubleshooting?

First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.

If there is still no product the PCR conditions might be too stringent and should be changed as follows:

• Lower the annealing temperature in 1 degree increments.
• Increase the extension time.
• Increase the amount of template added.

The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich. 

If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem.  Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.

Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?

No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on ultrataq PCR products, you can clean up the ultrataq PCR reaction first, then mix the cleaned-up ultrataq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the bestaq PCR products.

UltraTaq 2X PCR Master Mix - Dye

$168.00

Product Description

Biocrede’s Ultrataq DNA Polymerase is a gold standard in next-generation DNA polymerase enzymes. Now you can streamline your PCR with Biocrede’s Ultrataq 2x MasterMix with dye. Biocrede’s 2x PCR MasterMix with dye provides all of the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR process. This MasterMix comes with an inert blue dye and a stabilizer that allow for loading of the pcr products directly into an agarose gel, streamlining the process and reducing the risk of contamination.

Highlights
-Robust and high yield PCR across an array of templates.
-High processivity and shortened extension period.
-High fidelity, over 50x better than taq polymerase.
-Low cost per unit against comparable enzymes.
-Efficient amplification up to 16 kb.
-Robust amplification of AT and GC rich templates.

Application
-High-throughput PCR
-Amplification of long or difficult amplicons
-DNA cloning
-AT and GC rich templates
-RACE                            
-Template generation for NGS library construction
-Phylogenic Analysis

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Picture 1: Ultrataq DNA Polymerase successfully amplifies genomic template up to 15.6 kb.

Protocol  

Concentration: 5 U/μl

Storage: Store all components at -20 °C.Notes

Recommended products: UltraTaq master mix, UltraTaq master mix w/ dye, dNTP, Safe DNA stains

Notes: This product is distributed for laboratory research only.

tab3

How can I optimize the reaction for multiplexing?

The polymerase and Mg concentrations need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.

Is UltraTaq DNA Polymerase compatible with Uracil?

UltraTaq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.

If no amplification products are obtained, what parameters should be considered first when troubleshooting?

First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.

If there is still no product the PCR conditions might be too stringent and should be changed as follows:

• Lower the annealing temperature in 1 degree increments.
• Increase the extension time.
• Increase the amount of template added.

The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich. 

If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem.  Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.

Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?

No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on UltraTaq PCR products, you can clean up the UltrTaq PCR reaction first, then mix the cleaned-up UltraTaq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the UltraTaq PCR products.

UltraTaq DNA Polymerase

$84.00

Product Description

Looking to improve your routine PCR? UltraTaq DNA Polymerase is the new standard. With outstanding PCR yield, exceptional fidelity and high processivity, Biocrede’s UltraTaq DNA Polymerase is a versatile enzyme ideal for all PCR applications. With this superior enzyme, you can consolidate all PCR protocols and reactions into one efficient system.

With Biocrede’s UltraTaq DNA Polymerase, you can expect

• High-speed PCR without compromising accuracy
• High-processivity to reduce reaction time by 70%
• Robust and high yield across a wide range of templates
• Efficient amplification of DNA templates up to 15kb

Form	Enzyme supplied with 5X buffer, MgSO4 and 5X GC Enhancer
Picture 1	Ultrataq DNA Polymerase successfully amplifies genomic template up to 15.6 kb.
Picture 2	DNA Polymerase Enzyme Selection Guide  Polymerase Target Length Extension Speed (Per min.) Fidelity (vs. Taq) Proofreading MasterMix Ultrataq 15 kb 3-4,000 bp 50x Exactitaq 6 kb 1,000 bp 60x Taq 6 kb 1,000 bp 1x Taq Plus 6 kb 1,000 bp 5x Taq Express 12 kb 4-6,000 bp 10x Hottaq 6 kb 1,000 bp 1x Infinitaq 11 kb 4-6,000 bp 10x Hawkeye 20 kb 3-4,000 bp 1x Hemotaq 1 kb 1,000 bp 1x
Protocol
Concentration	5 U/μl
Storage	Store all components at -20 °C.
Notes	This product is distributed for laboratory research only. Caution: Not for diagnostic use .

tab3

How can I optimize the reaction for multiplexing?

The polymerase and Mg concentrations need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.

Is Ultrataq DNA Polymerase compatible with Uracil?

Ultrataq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.

If no amplification products are obtained, what parameters should be considered first when troubleshooting?

First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.

If there is still no product the PCR conditions might be too stringent and should be changed as follows:

• Lower the annealing temperature in 1 degree increments.
• Increase the extension time.
• Increase the amount of template added.

The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich. 

If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem.  Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.

Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?

No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on ultrataq PCR products, you can clean up the UltraTaq PCR reaction first, then mix the cleaned-up ultrataq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the bestaq PCR products.

UltraTaq DNA Polymerase

$84.00

Product Description

Looking to improve your routine PCR? UltraTaq DNA Polymerase is the new standard. With outstanding PCR yield, exceptional fidelity and high processivity, Biocrede’s UltraTaq DNA Polymerase is a versatile enzyme ideal for all PCR applications. With this superior enzyme, you can consolidate all PCR protocols and reactions into one efficient system.

With Biocrede’s UltraTaq DNA Polymerase, you can expect

• High-speed PCR without compromising accuracy
• High-processivity to reduce reaction time by 70%
• Robust and high yield across a wide range of templates
• Efficient amplification of DNA templates up to 15kb

Picture 1: Ultrataq DNA Polymerase successfully amplifies genomic template up to 15.6 kb.

Protocol   

Concentration: 5 U/μl

Storage: Store all components at -20 °C.Notes

Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains

Ultrataq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.

If no amplification products are obtained, what parameters should be considered first when troubleshooting?

First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.

If there is still no product the PCR conditions might be too stringent and should be changed as follows:

• Lower the annealing temperature in 1 degree increments.
• Increase the extension time.
• Increase the amount of template added.

The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich. 

If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem.  Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.

Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?

No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on ultrataq PCR products, you can clean up the ultrataq PCR reaction first, then mix the cleaned-up ultrataq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the bestaq PCR products.