ExactiTaq 2X PCR Master Mix

$138.00

Product Description

Biocrede's Exactitaq is an industry-leading high-fidelity DNA polymerase.  Biocrede's 2X PCR MasterMix provides all the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Exactitaq is the ideal choice for PCR that requires increased accuracy like cloning and sequencing, eliminating the need for downstream error correction steps. The use of Exactitaq DNA Polymerase for rare DNA templates is necessary as small amounts of DNA are especially prone to high mutant frequencies. 

Application

• Whole genome sequencing
• Site-directed mutagenesis
• Blunt-end cloning
• Specific amplification of difficult templates (i.e. GC-Rich)
• Low copy PCR assays

Form 5 x 1.0 ml vials

Concentration 2x

Storage Store all components at -20 °C.

Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

Protocol  

ExactiTaq 2X PCR Master Mix - Dye

$138.00

Description

Biocrede's Exactitaq is an industry-leading high-fidelity DNA polymerase.  Biocrede's 2X PCR MasterMix provides all the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Exactitaq is the ideal choice for PCR that requires increased accuracy like cloning and sequencing, eliminating the need for downstream error correction steps. The use of Exactitaq DNA Polymerase for rare DNA templates is necessary as small amounts of DNA are especially prone to high mutant frequencies. Precision MasterMix with dye contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region.

Application

• Whole genome sequencing
• Site-directed mutagenesis
• Blunt-end cloning
• Specific amplification of difficult templates (i.e. GC-Rich)
• Low copy PCR assays

Form 5 x 1.0 ml vials

Concentration 2x

Storage Store all components at -20 °C.

Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

Protocol 

HemoTaq 2X PCR Master Mix

$198.00

Description

Biocrede's Hemotaq DNA Polymerase 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. This enzyme is capable of performing PCR in reactions containing up to 20% (v/v) whole blood sample without a separate DNA purification step. As a result, the use of Biocrede's Hemotaq DNA Polymerase offers an overall reduction in contamination risk, experimental run time, and cost of genetic testing.

Applications

-Whole blood PCR

Form 5 x 1.0 ml vials

Concentration 2x

Storage Store all components at -20 °C.

Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

Protocol  

HiTaq 2X PCR Master Mix

$168.00

Product Description

Biocrede's HiTaq DNA Polymerase is a novel DNA polymerase with strategically engineered mutations resulting in a robust, high-fidelity polymerase. HiTaq DNA polymerase has exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR.

 Biocrede's HiTaq 2X PCR MasterMix is a ready-to-use mixture containing all the necessary reagents for highly successful amplification of DNA (high-fidelity DNA Polymerase, deoxynucleotides, reaction buffer and a sophisticated blend of additives in a 2X concentration). The HiTaq 2X PCR MasterMix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. HiTaq 2X PCR MasterMix is the most robust PCR MasterMix commercially available and will deliver an exceptional product yield with high fidelity.

 HiTaq 2X PCR MasterMix with dye contains an inert green dye mixture and a stabilizer which allow for direct loading of the final PCR products onto an agarose gel for analysis. The green dye resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of the gel electrophoresis, and the yellow band indicates the migrating front of the gel.

Features and Benefits

• Saves preparation time by combining DNA Polymerase, dNTPs and reaction buffer in a ready-to-use 2X mixture.
• Robust PCR performance with high yield and superb fidelity.
• Compatible with plant DNA templates.
• Reduces the risk of contamination by decreasing the number of pipetting steps.
• Provides consistent performance and results.

Protocol  

HiTaq 2X PCR Master Mix - Dye

$168.00

Product Description

Biocrede's HiTaq DNA Polymerase is a novel DNA polymerase with strategically engineered mutations resulting in a robust, high-fidelity polymerase. HiTaq DNA polymerase has exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This novel enzyme has intrinsically high processivity and is engineered to have an improved binding affinity for DNA resulting in highly successful PCR.

 Biocrede's HiTaq 2X PCR MasterMix is a ready-to-use mixture containing all the necessary reagents for highly successful amplification of DNA (high-fidelity DNA Polymerase, deoxynucleotides, reaction buffer and a sophisticated blend of additives in a 2X concentration). The HiTaq 2X PCR MasterMix advanced buffer system not only tolerates A/T- and G/C-rich content, but also many PCR inhibitors commonly found in a typical DNA sample. HiTaq 2X PCR MasterMix is the most robust PCR MasterMix commercially available and will deliver an exceptional product yield with high fidelity.

 HiTaq 2X PCR MasterMix with dye contains an inert green dye mixture and a stabilizer which allow for direct loading of the final PCR products onto an agarose gel for analysis. The green dye resolves during gel electrophoresis into a turquoise band at ~4000 bp and a yellow band at the ~50 bp region. The resolving bands of dye allow easy visualization of the real time progress of the gel electrophoresis, and the yellow band indicates the migrating front of the gel.

Features and Benefits

• Saves preparation time by combining DNA Polymerase, dNTPs and reaction buffer in a ready-to-use 2X mixture.
• Robust PCR performance with high yield and superb fidelity.
• Compatible with plant DNA templates.
• Reduces the risk of contamination by decreasing the number of pipetting steps.
• Provides consistent performance and results.

Protocol  

HotTaq 2X PCR Master Mix

$180.00

Product Description

The 2X PCR HotTaq MasterMix with dye contains, in addition, an inert green dye blend and a stabilizer which allows for direct loading of the final products onto a gel for analysis. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.

Features and Benefits

• Saves preparation time by combining HotTaq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture.
• Reduces the risk of contamination by decreasing the number of pipetting steps.
• Provides consistent reaction performance and results.

Protocol  

HotTaq 2X PCR Master Mix- Dye

$180.00

Product Description

The 2X PCR HotTaq MasterMix with dye contains, in addition, an inert green dye blend and a stabilizer which allows for direct loading of the final products onto a gel for analysis. The green dye blend resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region. The resolving bands of dye allow easy visualization of the real time progress of your gel electrophoresis, where the yellow band indicates the migrating front in the gel.

Features and Benefits

• Saves preparation time by combining HotTaq DNA Polymerase, dNTPs and reaction buffer in a ready-to-use mixture.
• Reduces the risk of contamination by decreasing the number of pipetting steps.
• Provides consistent reaction performance and results.

Protocol 

One-Step RT-PCR Kit

$195.00

Product Description

One reaction tube for RT and PCR now offers unparalleled sensitivity and efficiency. With Biocrede’s  One-Step RT-PCR Kit, you can streamline the procedure for reverse transcription and subsequent PCR in the same reaction tube, eliminating the need to change buffers and reducing the risk of contamination. This kit offers the end-user an efficient and easy alternative to the conventional “two-step” RT-PCR approach with extreme sensitivity, specificity and high product yield.

PCR-2010-dye buffer contains Cresol Red as an electrophoresis dye, with migration equivalent to 125 bp DNA fragment on an agarose gel.

Application

• Gene-expression analysis
• Transcription analysis
• Gene cloning
• Multiplex RT-PCR
• High throughput applications
• Virus detection and quantification

Protocol  

Taq 2X PCR MasterMix

$72.00

Product Description

Taq 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, Biocrede offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. Biocrede's MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.

Application

• Routine PCR amplification of DNA templates up to 6 kb
• Suitable for a wide range of PCR assays
• TA cloning

 Protocol  

Taq Plus 2X PCR Master Mix

$96.00

Production Description

Streamline your PCR with Biocrede’s 2X PCR MasterMix. Biocrede’s 2X PCR MasterMix provides all ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR workflow. Utilizing different Taq DNA Polymerases, Biocrede offers a variety of highly sensitive MasterMixes to accommodate a wide range of DNA templates and performance needs. Biocrede’s MasterMix with dye includes, in addition, an inert blue dye and a stabilizer which allow for direct loading of the PCR product(s) onto an agarose gel.

 The use of Taq DNA Polymerase in this MasterMix offers:

• Robust PCR performance with great reproducibility
• High sensitivity

Application

• Routine PCR amplification of DNA templates up to 6 kb
• Suitable for a wide range of PCR assays
• TA cloning

Protocol  

tab2

Storage: Store all components at -20 °C.

Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains

Notes: This product is distributed for laboratory research only. dNTP sold seperately.

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TaqExpress 2X PCR Master Mix

$108.00

Product Description

With extension rate 6x faster than taq polymerase, Biocrede's TaqExpress 2X PCR MasterMix provides all the necessary ingredients for PCR in a premixed and optimized format that streamlines the PCR workflow.  In addition to increased throughput, TaqExpress DNA polymerase also possesses moderate 3’-5’ proofreading activity, making this enzyme well suited for high-throughput PCR.  Biocrede's TaqExpress is the solution to your fast and high-throughput PCR needs.

Applications

- Fast PCR

- High-throughput PCR

Form 5 x 1.0 ml vials

Concentration 2x

Storage Store all components at -20 °C.

Protocol  

Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

TaqExpress 2X PCR Master Mix- Dye

$108.00

Product Description

With extension rate 6x faster than taq polymerase Biocrede's TaqExpress 2X PCR MasterMix provides all the necessary ingredients for PCR in a premixed and optimized format that streamlines the PCR workflow.  In addition to increased throughput, TaqFast DNA polymerase also possesses moderate 3’-5’ proofreading activity, making this enzyme well suited for high-throughput PCR.  Biocrede's TaqExpress contains a green dye blend which resolves during gel electrophoresis into a turquoise band at ~4000bp and a yellow band at the ~50bp region.

Application

- Fast PCR
- High-throughput PCR

Form 5 x 1.0 ml vials

Concentration 2x

Storage Store all components at -20 °C.

Notes This product is distributed for laboratory research only.
Caution: Not for diagnostic use.

tab3

UltraTaq 2X PCR Master Mix

$168.00

Product Description

Biocrede’s Ultrataq DNA Polymerase is a gold standard in next-generation DNA polymerase enzymes. Now you can streamline your PCR process with Biocrede’s Ultrataq 2x MasterMix. Biocrede’s 2x PCR MasterMix provides all of the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR process. Biocrede offers an array of MasterMixes to accommodate every PCR need. We also offer a line of MasterMixes that come with an inert blue dye and a stabilizer that allow for loading of the pcr products directly into an agarose gel.

Highlights
-Robust and high yield PCR across an array of templates.
-High processivity and shortened extension period.
-High fidelity, over 50x better than taq polymerase.
-Low cost per unit against comparable enzymes.
-Efficient amplification up to 16 kb.
-Robust amplification of AT and GC rich templates.

Application
-High-throughput PCR
-Amplification of long or difficult amplicons
-DNA cloning
-AT and GC rich templates
-RACE                            
-Template generation for NGS library construction
-Phylogenic Analysis

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Picture 1: Ultrataq DNA Polymerase successfully amplifies genomic template up to 15.6 kb.

. 

Protocol  

Concentration: 2x

Storage: Store all components at -20 °C.Notes

Recommended products: Ultrataq master mix, Ultrataq master mix w/ dye, dNTP, Safe DNA stains

Notes: This product is distributed for laboratory research only.

tab3

How can I optimize the reaction for multiplexing?

The polymerase and Mg concentrations need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.

Is Ultrataq DNA Polymerase compatible with Uracil?

Ultrataq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.

If no amplification products are obtained, what parameters should be considered first when troubleshooting?

First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.

If there is still no product the PCR conditions might be too stringent and should be changed as follows:

• Lower the annealing temperature in 1 degree increments.
• Increase the extension time.
• Increase the amount of template added.

The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich. 

If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem.  Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.

Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?

No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on ultrataq PCR products, you can clean up the ultrataq PCR reaction first, then mix the cleaned-up ultrataq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the bestaq PCR products.

UltraTaq 2X PCR Master Mix - Dye

$168.00

Product Description

Biocrede’s Ultrataq DNA Polymerase is a gold standard in next-generation DNA polymerase enzymes. Now you can streamline your PCR with Biocrede’s Ultrataq 2x MasterMix with dye. Biocrede’s 2x PCR MasterMix with dye provides all of the ingredients necessary for PCR in a premixed and optimized format that simplifies the PCR process. This MasterMix comes with an inert blue dye and a stabilizer that allow for loading of the pcr products directly into an agarose gel, streamlining the process and reducing the risk of contamination.

Highlights
-Robust and high yield PCR across an array of templates.
-High processivity and shortened extension period.
-High fidelity, over 50x better than taq polymerase.
-Low cost per unit against comparable enzymes.
-Efficient amplification up to 16 kb.
-Robust amplification of AT and GC rich templates.

Application
-High-throughput PCR
-Amplification of long or difficult amplicons
-DNA cloning
-AT and GC rich templates
-RACE                            
-Template generation for NGS library construction
-Phylogenic Analysis

tab2

Picture 1: Ultrataq DNA Polymerase successfully amplifies genomic template up to 15.6 kb.

Protocol  

Concentration: 5 U/μl

Storage: Store all components at -20 °C.Notes

Recommended products: UltraTaq master mix, UltraTaq master mix w/ dye, dNTP, Safe DNA stains

Notes: This product is distributed for laboratory research only.

tab3

How can I optimize the reaction for multiplexing?

The polymerase and Mg concentrations need to be optimized in multiplexing and this will need to be evaluated per case by the end user. Primer concentrations for each amplicon in multiplexing would also need to be optimized. The general rule would be lowering the primer concentrations of the strongly amplified targets and increasing the primer concentrations of the weakly amplified targets.

Is UltraTaq DNA Polymerase compatible with Uracil?

UltraTaq DNA polymerase is unable to incorporate dUTP and read through uracil present in DNA templates.

If no amplification products are obtained, what parameters should be considered first when troubleshooting?

First, rerun PCR paying attention that all PCR components are functional and added in the proper volume.

If there is still no product the PCR conditions might be too stringent and should be changed as follows:

• Lower the annealing temperature in 1 degree increments.
• Increase the extension time.
• Increase the amount of template added.

The next most common reasons for PCR failure are the presence of PCR inhibitors in the template DNA and a template or amplicon that is G-C rich. These problems can be mitigated by diluting the template and the addition of our GC enhancer in increments of 1 ul per 50 ul reaction if the target amplicon is G-C rich. 

If none of these troubleshooting techniques resolve the issue then the quality or design of the primers might be the problem.  Try using different amounts of primer in solution or reordering/ redesigning new primers and re-amplifying the amplicon.

Does this enzyme have terminal transferase activity (i.e. adds an A at the end of the PCR product)?

No, all high fidelity polymerases will not have this feature. If you would like to do T/A cloning on UltraTaq PCR products, you can clean up the UltrTaq PCR reaction first, then mix the cleaned-up UltraTaq PCR product with Taq/PCR buffer/dNTP, and incubate it for 15-30 mins. The Taq will add the dA overhangs onto the UltraTaq PCR products.